Anti-TIGAR [M2-P4H2]
Invented by Ayham Alnabulsi from Vertebrate Antibodies Limited
Invented at Vertebrate Antibodies Limited
- Datasheet
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Info
![Immunohistochemistry of formalin-fixed, paraffin-embedded human colorectal cancer tissue using anti-TIGAR [M2-P4H2] showing strong cytoplasmic and nuclear staining.](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,q_auto/4d6c83a5-03d9-48cd-86dc-3c357a0c06d5.jpg)
![Immunohistochemistry of formalin-fixed, paraffin-embedded human colorectal cancer tissue using anti-TIGAR [M2-P4H2] showing strong cytoplasmic and nuclear staining.](https://res.cloudinary.com/ximbio/image/upload/c_limit,fl_lossy,h_282,q_auto,w_368/4d6c83a5-03d9-48cd-86dc-3c357a0c06d5.jpg)
![Image thumbnail for Anti-TIGAR [M2-P4H2]](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,h_45,q_auto/085c53eb-352c-4891-bc51-c291ac693d6f.jpg)
![Image thumbnail for Anti-TIGAR [M2-P4H2]](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,h_45,q_auto/c49fd127-af63-48cf-a637-68dd2812e078.jpg)
![Image thumbnail for Anti-TIGAR [M2-P4H2]](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,h_45,q_auto/6f4502f4-a528-47b3-b8c4-ef57ff106106.jpg)
![Image thumbnail for Anti-TIGAR [M2-P4H2]](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,h_45,q_auto/4d6c83a5-03d9-48cd-86dc-3c357a0c06d5.jpg)
| Catalogue Number | 151870 |
| Applications | ELISA IHC IF WB |
| Antigen/Gene or Protein Targets | TP53-induced glycolysis and apoptosis regulator (TIGAR) |
| Reactivity | Human |
| Relevance |
TIGAR (TP53 (tumor protein 53)-induced glycolysis and apoptosis regulator), also known as C12orf5, is a 270 amino acid protein induced by the p53 tumor suppressor pathway that functions to protect against oxidative stress. TIGAR shares sequence similarity with the bisphosphate domain of the fructose- 2,6-bisphosphate degrading enzyme (fructose bisphosphatase or FBPase) of the glycolysis pathway and can thus lower the intracellular levels of fructose- 2,6-bisphosphate. TIGAR specifically functions to block glycolysis, leading the pathway to the pentose phosphate shunt and decreasing the intracellular concentration of reactive oxygen species. This suggests a role for TIGAR in protecting cells from reactive oxygen species that can be DNA damaging and lead to apoptosis. |
| Host | Mouse |
| Immunogen | Synthetic peptide; IINFEEGREV (amino acids 239-248) |
| Positive Control | ELISA- peptide immunogenWestern Blot- Hela Whole cell extract IF- Hela cellsIHC- Human colon carcinoma |
| Subclass | IgG1 kappa |
| Myeloma Used | P3X63Ag8.653 |
| Recommended Growing Conditions | Dulbecco’s media containing 20% Fetal Bovine serum (DH20) prepared as follows (for final volume of 300ml: 237ml DMEM + 60 ml Fetal Bovine Serum plus 3ml L-Glutamine(Sigma G7513) + 3ml of Penicillin -Streptomycin (Sigma P0781). |
| Strain | Balb/c |
| Research Area | Apoptosis and Programmed Cell Death, Cancer, DNA Damage and Repair |
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