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Anti-TIGAR [M2-P4H2]

Invented by Ayham Alnabulsi at Vertebrate Antibodies Limited

Info

Catalogue Number 151870
Applications ELISA IHC IF WB
Antigen/Gene or Protein Targets TP53-induced glycolysis and apoptosis regulator (TIGAR)
Reactivity Human
Relevance TIGAR (TP53 (tumor protein 53)-induced glycolysis and apoptosis regulator),
also known as C12orf5, is a 270 amino acid protein induced by the p53 tumor
suppressor pathway that functions to protect against oxidative stress. TIGAR
shares sequence similarity with the bisphosphate domain of the fructose-
2,6-bisphosphate degrading enzyme (fructose bisphosphatase or FBPase) of
the glycolysis pathway and can thus lower the intracellular levels of fructose-
2,6-bisphosphate. TIGAR specifically functions to block glycolysis, leading
the pathway to the pentose phosphate shunt and decreasing the intracellular
concentration of reactive oxygen species. This suggests a role for
TIGAR in protecting cells from reactive oxygen species that can be DNA
damaging and lead to apoptosis.
Host Mouse
Immunogen Synthetic peptide; IINFEEGREV (amino acids 239-248)
Subclass IgG1 kappa
Positive Control ELISA- peptide immunogenWestern Blot- Hela Whole cell extract IF- Hela cellsIHC- Human colon carcinoma
Myeloma Used P3X63Ag8.653
Recommended Growing Conditions Dulbecco’s media containing 20% Fetal Bovine serum (DH20) prepared as follows (for final volume of 300ml: 237ml DMEM + 60 ml Fetal Bovine Serum plus 3ml L-Glutamine(Sigma G7513) + 3ml of Penicillin -Streptomycin (Sigma P0781).
Strain Balb/c
Research Area Apoptosis and Programmed Cell Death, Cancer, DNA Damage and Repair

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