- Reviews (0)
|Applications||ELISA IF Fn WB|
|Antigen/Gene or Protein Targets||Human Respiratory Syncytial (RS) virus G glycoprotein|
Human Respiratory Syncytial Virus (RSV) is a major cause of lower respiratory tract illness and is the chief cause of hospitalization for respiratory tract illness in young children.
The glycoprotein G (also named GP90) is located on the surface of viral envelope, its function is to attach to host cell receptors. It has high content (90%) of carbohydrate.
To our knowledge, this is the only G specific antibody reported to induce in vitro antibody-dependent enhancement of macrophage cells (Clin Diagn Lab Immunol. 1996 May;3(3):280-6).
|Immunogen||Gradient-purified RSF-44 virus (subgroup A) UV inactivated for 20 minutes at 20C|
|Positive Control||WB: Ag: gradient-purified RS virus (see figure). IF: staining of RSA-2 infected BSC-1 cells|
|Recommended Growing Conditions||Dulbecco’s media containing 20% Fetal Bovine serum (DH20) prepared as follows (for final volume of 300ml: 237ml DMEM plus 60 ml Fetal Bovine Serum plus 3ml L-Glutamine).|
|Notes||Antibody reactive with Glycoprotein G of subgroup B|
Have you used this reagent? Share your experience by submitting a review and help your fellow scientists.Review this reagent