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Anti-RSV F Glycoprotein [11-2-C9]

Invented by Ayham Alnabulsi at Vertebrate Antibodies Limited

Info

Catalogue Number 151856
Applications ELISA IF Fn WB
Antigen/Gene or Protein Targets Human Respiratory Syncytial (RS) virus Fusion glycoprotein
Reactivity Virus
Relevance Human Respiratory Syncytial Virus (RSV) is a major cause of lower respiratory tract illness and is the chief cause of hospitalization for respiratory tract illness in young children.The glycoprotein F is located on the surface of viral envelope, its function is to induce fusion of viral envelope with host-cell envelope resulting in syncytium formation. The glycoprotein F (also named VP70, F0 or fusion protein) consists of two components: F1 (also named VPG48) and F2 (also named VGP26) held together by disulphide bonds. The reported molecular weight of the VGP26 component varies between 20 to 26 kDa.
Host Mouse
Immunogen Gradient-purified RSF-44 virus (subgroup A) UV inactivated for 20 minutes at 20C
Subclass IgG1 kappa
Positive Control Immunoblot: Ag: gradient-purified RS virus (see figure). Indirect immunofluorescence: staining of RSA-2 infected BSC-1 cells
Molecular Weight (kDa) The identity and molecular weight of the protein target of this antibody was validated by including within the immunoblot assay (as a marker) a convalescent serum sample from a RS virus infected patient. The protein specificities of the antibodies induced in the human convalescent serum is described in Gimenez et al. (1987).
Myeloma Used P3X63Ag8.653
Recommended Growing Conditions Dulbecco’s media containing 20% Foetal Bovine serum and 2mM Glutamine.
Strain Balb/c
Notes Immunoblot using the reduced and unreduced RS virus shows that 11-2-C9 reacts with the non-reduced form of the virus F protein (VP70). It has a plaque reduction neutralization titer of 10 2.

This antibody reacts with the fusion glycoprotein, F (also named VP70 or F0 protein), from both subgroups A and B of RS virus.

The 11-2-C9 antibody has RS virus enhancing activity in vitro (enhancement index range: 4.3-5.6) (Gimenez et al., 1996).
Research Area Virology

References: 3 entries

Gimenez et al. 1996. Clin Diagn Lab Immunol. 3(3):280-6. PMID: 8705669.

Gimenez et al. 1986. J Gen Virol. 67 ( Pt 5):863-70. PMID: 3517224.

Fn

Gimenez et al. 1984. J Gen Virol. 65 ( Pt 5):963-71. PMID: 6202832.


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References: 3 entries

Gimenez et al. 1996. Clin Diagn Lab Immunol. 3(3):280-6. PMID: 8705669.

Gimenez et al. 1986. J Gen Virol. 67 ( Pt 5):863-70. PMID: 3517224.

Fn

Gimenez et al. 1984. J Gen Virol. 65 ( Pt 5):963-71. PMID: 6202832.


Add a reference

References: 3 entries

Gimenez et al. 1996. Clin Diagn Lab Immunol. 3(3):280-6. PMID: 8705669.

Gimenez et al. 1986. J Gen Virol. 67 ( Pt 5):863-70. PMID: 3517224.

Fn

Gimenez et al. 1984. J Gen Virol. 65 ( Pt 5):963-71. PMID: 6202832.


Add a reference


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