- Reviews (0)
|Applications||ELISA IF Fn WB|
|Antigen/Gene or Protein Targets||Human Respiratory Syncytial (RS) virus Fusion glycoprotein|
|Relevance||Human Respiratory Syncytial Virus (RSV) is a major cause of lower respiratory tract illness and is the chief cause of hospitalization for respiratory tract illness in young children.The glycoprotein F is located on the surface of viral envelope, its function is to induce fusion of viral envelope with host-cell envelope resulting in syncytium formation. The glycoprotein F (also named VP70, F0 or fusion protein) consists of two components: F1 (also named VPG48) and F2 (also named VGP26) held together by disulphide bonds. The reported molecular weight of the VGP26 component varies between 20 to 26 kDa.|
|Immunogen||Gradient-purified RSF-44 virus (subgroup A) UV inactivated for 20 minutes at 20C|
|Positive Control||Immunoblot: Ag: gradient-purified RS virus (see figure). Indirect immunofluorescence: staining of RSA-2 infected BSC-1 cells|
|Molecular Weight (kDa)||The identity and molecular weight of the protein target of this antibody was validated by including within the immunoblot assay (as a marker) a convalescent serum sample from a RS virus infected patient. The protein specificities of the antibodies induced in the human convalescent serum is described in Gimenez et al. (1987).|
|Recommended Growing Conditions||Dulbecco’s media containing 20% Foetal Bovine serum and 2mM Glutamine.|
Immunoblot using the reduced and unreduced RS virus shows that 11-2-C9 reacts with the non-reduced form of the virus F protein (VP70). It has a plaque reduction neutralization titer of 10 2.
This antibody reacts with the fusion glycoprotein, F (also named VP70 or F0 protein), from both subgroups A and B of RS virus.
The 11-2-C9 antibody has RS virus enhancing activity in vitro (enhancement index range: 4.3-5.6) (Gimenez et al., 1996).
Have you used this reagent? Share your experience by submitting a review and help your fellow scientists.Review this reagent