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Anti-RSV F Glycoprotein [4-15]

Invented by Ayham Alnabulsi at Vertebrate Antibodies Limited

Info

Catalogue Number 151854
Applications ELISA IHC IF WB
Antigen/Gene or Protein Targets Respiratory Syncytial (RS) virus Fusion glycoprotein
Reactivity Virus
Relevance Human Respiratory Syncytial Virus (RSV) is a major cause of lower respiratory tract illness and is the chief cause of hospitalization for respiratory tract illness in young children.The glycoprotein F is located on the surface of viral envelope, its function is to induce fusion of viral envelope with host-cell envelope resulting in syncytium formation. The glycoprotein F (also named VP70, F0 or fusion protein) consists of two components: F1 (also named VPG48) and F2 (also named VGP26) held together by disulphide bonds. The reported molecular weight of the VGP26 component varies between 20 to 26 kDa
Host Mouse
Immunogen The immunogen was gradient purified and UV inactivated RSN2 virus (subgroup B). The procedure used to produce this antibody is described in Gimenez et al. (1984).
Subclass IgG1
Positive Control Immunoblot: Gradient-purified RSN-2 virus 5ug per lane (see figure) Indirect immunofluorescence: staining of live unfixed RSN-2 infected BSC-1 cells (see figure)
Molecular Weight (kDa) The identity and molecular weight of the protein target of this antibody was validated by including within the immunoblot assay (as a marker) a convalescent serum sample from a RS virus infected patient. The protein specificities of the antibodies induced in the human convalescent serum is described in Gimenez et al. (1987).
Myeloma Used P3X63Ag8.653
Recommended Growing Conditions Dulbecco’s media containing 20% Fetal Bovine serum (DH20) prepared as follows (for final volume of 300ml: 237ml DMEM plus 60 ml Fetal Bovine Serum plus 3ml L-Glutamine).
Strain Balb/c
Notes This antibody reacts with the fusion glycoprotein F (also named VP70 or F0 protein) from both RS virus subgroups A and B.

On the basis of the reactivity of this antibody and both the antibodies clone 4-14 (cat number: 151851) and clone 3-5-18 (cat number: 151850) the existence of two antigenic types of human RS virus was identified, Gimenez et al. (1986).
Research Area Virology

References: 3 entries

Benedicenti et al. 2015. Fish Shellfish Immunol. 46(2):417-25. PMID: 26166456.

IHC

Gimenez et al. 1986. J Gen Virol. 67 ( Pt 5):863-70. PMID: 3517224.

Gimenez et al. 1986. J Gen Virol. 67 ( Pt 5):863-70. PMID: 3517224.


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References: 3 entries

Benedicenti et al. 2015. Fish Shellfish Immunol. 46(2):417-25. PMID: 26166456.

IHC

Gimenez et al. 1986. J Gen Virol. 67 ( Pt 5):863-70. PMID: 3517224.

Gimenez et al. 1986. J Gen Virol. 67 ( Pt 5):863-70. PMID: 3517224.


Add a reference

References: 3 entries

Benedicenti et al. 2015. Fish Shellfish Immunol. 46(2):417-25. PMID: 26166456.

IHC

Gimenez et al. 1986. J Gen Virol. 67 ( Pt 5):863-70. PMID: 3517224.

Gimenez et al. 1986. J Gen Virol. 67 ( Pt 5):863-70. PMID: 3517224.


Add a reference


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