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|Applications||ELISA IHC IF WB|
|Antigen/Gene or Protein Targets||Respiratory Syncytial (RS) virus Fusion glycoprotein|
|Relevance||Human Respiratory Syncytial Virus (RSV) is a major cause of lower respiratory tract illness and is the chief cause of hospitalization for respiratory tract illness in young children.The glycoprotein F is located on the surface of viral envelope, its function is to induce fusion of viral envelope with host-cell envelope resulting in syncytium formation. The glycoprotein F (also named VP70, F0 or fusion protein) consists of two components: F1 (also named VPG48) and F2 (also named VGP26) held together by disulphide bonds. The reported molecular weight of the VGP26 component varies between 20 to 26 kDa|
|Immunogen||The immunogen was gradient purified and UV inactivated RSN2 virus (subgroup B). The procedure used to produce this antibody is described in Gimenez et al. (1984).|
|Positive Control||Immunoblot: Gradient-purified RSN-2 virus 5ug per lane (see figure) Indirect immunofluorescence: staining of live unfixed RSN-2 infected BSC-1 cells (see figure)|
|Molecular Weight (kDa)||The identity and molecular weight of the protein target of this antibody was validated by including within the immunoblot assay (as a marker) a convalescent serum sample from a RS virus infected patient. The protein specificities of the antibodies induced in the human convalescent serum is described in Gimenez et al. (1987).|
|Recommended Growing Conditions||Dulbecco’s media containing 20% Fetal Bovine serum (DH20) prepared as follows (for final volume of 300ml: 237ml DMEM plus 60 ml Fetal Bovine Serum plus 3ml L-Glutamine).|
This antibody reacts with the fusion glycoprotein F (also named VP70 or F0 protein) from both RS virus subgroups A and B.
On the basis of the reactivity of this antibody and both the antibodies clone 4-14 (cat number: 151851) and clone 3-5-18 (cat number: 151850) the existence of two antigenic types of human RS virus was identified, Gimenez et al. (1986).
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