Anti-RSV Subgroup B Phosphoprotein [4-14]
Invented by Ayham Alnabulsi from Vertebrate Antibodies Limited
Invented at Vertebrate Antibodies Limited
- Datasheet
- References (6)
- Inventor Info
Info
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| Catalogue Number | 151851 |
| Applications | ELISA IF WB |
| Antigen/Gene or Protein Targets | Human Respiratory Syncytial (RS) virus phosphoprotein VP32 |
| Reactivity | Virus |
| Relevance |
Immunoblot assays performed in parallel using as antigen 19 clinical RS virus isolates of subgroups A and B (isolated between 1972 and 1984) showed that this antibody reacted only with the phosphoprotein of RS virus subgroup B (Gimenez et al, 1986). RS virus subgroups A and B circulate concurrently in the human population. Infections with RS virus subgroup A are thought to produce more severe disease than infections with RS virus subgroup B. Consequently, it is beneficial for patient management to know early during an RS virus infection whether the patient is infected with RS virus subgroup A or B. Thus, this antibody is relevant for as a diagnostic tool to determine, soon after infection, the specific RS virus subgroup responsible for the RS virus infection. A diagnostic kit protocol is described in Notes. |
| Host | Mouse |
| Immunogen | The immunogen was gradient-purified RSN-2 virus (subgroup B) that was then treated with 0.1% SDS at 100°C for 2 min. The procedure used to produce this antibody is described in Gimenez et al. (1984). |
| Positive Control | Immunoblot: Partial purified PVM (lane 2). Partial purified BRS virus (lane 3). Gradient-purified RSN-2 virus (lane1 & 4): 5ug/lane. First antibody: 4-14 antibody (lanes 1,2,3 & 4). Indirect immunofluorescence: staining of RSN-2 infected BSC-1 cells |
| Subclass | IgM |
| Myeloma Used | P3X63Ag8.653 |
| Recommended Growing Conditions | Dulbecco’s media containing 20% Fetal Bovine serum (DH20) prepared as follows (for final volume of 300ml: 237ml DMEM plus 60 ml Fetal Bovine Serum plus 3ml L-Glutamine). |
| Strain | Balb/c |
| Notes |
Diagnostic kit proposal: Antibody : 3-5-18 4-14 Cat number : 151850 151851 Control samples: RS A2 Subgrp A + - RSN-2 Subgrp B + + Patient # 1 sample + - Patient # 2 sample + + Results: Patient # 1 was infected with RSV Subgrp A Patient # 2 was infected with RSV Subgrp B. |
| Research Area | Virology |
References: 6 entries
Gimenez et al. 1987. J Gen Virol. 68 ( Pt 5):1267-75. PMID: 3572364.
Immunoblot analysis of the human antibody response to respiratory syncytial virus infection.
Europe PMC ID: 3572364
Gimenez et al. 1986. J Gen Virol. 67 ( Pt 5):863-70. PMID: 3517224.
Antigenic variation between human respiratory syncytial virus isolates.
Europe PMC ID: 3517224
Gimenez et al. 1984. J Gen Virol. 65 ( Pt 5):963-71. PMID: 6202832.
Monoclonal antibodies to human respiratory syncytial virus and their use in comparison of different virus isolates.
Europe PMC ID: 6202832
Add a reference
References: 6 entries
Gimenez et al. 1987. J Gen Virol. 68 ( Pt 5):1267-75. PMID: 3572364.
Immunoblot analysis of the human antibody response to respiratory syncytial virus infection.
Gimenez et al. 1986. J Gen Virol. 67 ( Pt 5):863-70. PMID: 3517224.
Antigenic variation between human respiratory syncytial virus isolates.
Gimenez et al. 1984. J Gen Virol. 65 ( Pt 5):963-71. PMID: 6202832.
Monoclonal antibodies to human respiratory syncytial virus and their use in comparison of different virus isolates.
Add a reference
