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|Applications||ELISA IF WB|
|Antigen/Gene or Protein Targets||Human Respiratory Syncytial (RS) virus phosphoprotein VP32|
Immunoblot assays performed in parallel using as antigen 19 clinical RS virus isolates of subgroups A and B (isolated between 1972 and 1984) showed that this antibody reacted only with the phosphoprotein of RS virus subgroup B (Gimenez et al, 1986).
RS virus subgroups A and B circulate concurrently in the human population. Infections with RS virus subgroup A are thought to produce more severe disease than infections with RS virus subgroup B. Consequently, it is beneficial for patient management to know early during an RS virus infection whether the patient is infected with RS virus subgroup A or B. Thus, this antibody is relevant for as a diagnostic tool to determine, soon after infection, the specific RS virus subgroup responsible for the RS virus infection.
A diagnostic kit protocol is described in Notes.
|Immunogen||The immunogen was gradient-purified RSN-2 virus (subgroup B) that was then treated with 0.1% SDS at 100°C for 2 min. The procedure used to produce this antibody is described in Gimenez et al. (1984).|
|Positive Control||Immunoblot: Partial purified PVM (lane 2). Partial purified BRS virus (lane 3). Gradient-purified RSN-2 virus (lane1 & 4): 5ug/lane. First antibody: 4-14 antibody (lanes 1,2,3 & 4). Indirect immunofluorescence: staining of RSN-2 infected BSC-1 cells|
|Recommended Growing Conditions||Dulbecco’s media containing 20% Fetal Bovine serum (DH20) prepared as follows (for final volume of 300ml: 237ml DMEM plus 60 ml Fetal Bovine Serum plus 3ml L-Glutamine).|
Diagnostic kit proposal:
Antibody : 3-5-18 4-14
Cat number : 151850 151851
RS A2 Subgrp A + -
RSN-2 Subgrp B + +
Patient # 1 sample + -
Patient # 2 sample + +
Patient # 1 was infected with RSV Subgrp A Patient # 2 was infected with RSV Subgrp B.
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