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|Applications||ELISA IF WB|
|Antigen/Gene or Protein Targets||Human Respiratory Syncytial (RS) virus Phosphoprotein, VP32|
This antibody reacts with the phosphoprotein from both human RS virus subgroups A and B as well as the phosphoprotein of bovine RS virus. The antibody is not reactive with the phosphoprotein of other member of Pneumovirus genus (specifically, pneumonia virus of mice).
On the basis of the reactivity of this antibody and the 4-14 antibody (cat number: 151851) against the VP32 protein (by immunoblot and immunofluorescence) the existence of two antigenic types of human RS virus was identified (Gimenez et al., 1986).
The phosphoprotein is located within the virion nucleocapsid. The phosphoprotein of RS virus subgroup A is slightly larger than the phosphoprotein of RS virus subgroup B. This difference in molecular weight was demonstrated by immunoblot using this antibody (Gimenez et al., 1986).
This antibody is useful for the diagnosis of RS virus infection since the phosphoprotein is abundant which makes the detection assays using this antibody highly sensitive.
|Immunogen||The immunogen was gradient-purified RSN-2 virus (subgroup B) that was then treated with 0.1% SDS at 100°C for 2 min. The procedure used to produce this antibody is described in Gimenez et al. (1984).|
|Positive Control||Immunoblot: Gradient-purified RSN-2 virus 5ug per lane. Lanes 1 and 2.First antibodies: Lane 1: 3-5 antibody. Lane2: RSV convalescent human sera.Indirect immunofluorescence: staining of RSN-2 infected BSC-1 cells|
|Recommended Growing Conditions||Dulbecco’s media containing 20% Fetal Bovine serum (DH20) prepared as follows (for final volume of 300ml: 237ml DMEM plus 60 ml Fetal Bovine Serum plus 3ml L-Glutamine).|
|Notes||Not reactive with other member of genus Pneumovirus (Pneumonia virus of mice). Antibody reactive with VPP32 from human RSV of subgroup A and bovine respiratory syncytial virus (BRS)|
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