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Anti-ANO5 [5F7]

Info

Catalogue Number 151849
Applications IF WB
Antigen/Gene or Protein Targets Anoctamin 5
Reactivity Human, Mouse and Rat
Relevance Antibody to muscular dystrophy gene. Relevant for anoctamin 5 linked diseases - muscular dystrophy, GDD1, dysferlinopathy. Antibody is of use for diagnostic and research purposes on anoctamin 5 and other anoctamins. The antibody also has relevance in research in cell membrane repair, membrane trafficking and chloride channels.
Host Mouse
Immunogen Sythetic peptide, C-Terminal. "CKREKLMTIKILHDFE"
Subclass IgM
Positive Control C2C12, skeletal muscle
Molecular Weight (kDa) 37
Myeloma Used Sp2/0-Ag14
Recommended Growing Conditions DMEM + antibiotic + 10% FCS
Notes Molecular Weight: The protein has an expected molecular mass of ~107kDa. However, it is rarely detected using this antibody in muscle tissue. By western blot using lysates prepared from C2C12 cells (wt), RD and fibroblasts 3 bands are detected; 37 KDa, 55kDa and the expected molecular mass.

In the Aceview database (http://www.ncbi.nlm.nih.gov/IEB/Research/Acembly/av.cgi?db=human&term=ano5&submit=Go) there is evidence from clones of a short C-terminal isoform. This is likely the small isoform detected in control muscle.
Research Area Cardiovascular, Cell Signaling & Signal Transduction

References: 1 entry

Bashir et al. 2014. Neuromuscular Disorders. 24:1


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References: 1 entry

Bashir et al. 2014. Neuromuscular Disorders. 24:1


Add a reference

References: 1 entry

Bashir et al. 2014. Neuromuscular Disorders. 24:1


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