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|Antigen/Gene or Protein Targets||Anoctamin 5|
|Reactivity||Human, Mouse and Rat|
|Relevance||Antibody to muscular dystrophy gene. Relevant for anoctamin 5 linked diseases - muscular dystrophy, GDD1, dysferlinopathy. Antibody is of use for diagnostic and research purposes on anoctamin 5 and other anoctamins. The antibody also has relevance in research in cell membrane repair, membrane trafficking and chloride channels.|
|Immunogen||Sythetic peptide, C-Terminal. "CKREKLMTIKILHDFE"|
|Positive Control||C2C12, skeletal muscle|
|Molecular Weight (kDa)||37|
|Recommended Growing Conditions||DMEM + antibiotic + 10% FCS|
Molecular Weight: The protein has an expected molecular mass of ~107kDa. However, it is rarely detected using this antibody in muscle tissue. By western blot using lysates prepared from C2C12 cells (wt), RD and fibroblasts 3 bands are detected; 37 KDa, 55kDa and the expected molecular mass.
In the Aceview database (http://www.ncbi.nlm.nih.gov/IEB/Research/Acembly/av.cgi?db=human&term=ano5&submit=Go) there is evidence from clones of a short C-terminal isoform. This is likely the small isoform detected in control muscle.
|Research Area||Cardiovascular, Cell Signaling & Signal Transduction|
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