MEF OGG1 KO Cell Line
Invented at Cancer Research UK London Research Institute: Clare Hall Laboratories
- Datasheet
- References (2)
- Inventor Info
Info
| Catalogue Number | 151846 |
| Antigen/Gene or Protein Targets | OGG1 |
| Host | Mouse |
| Tissue | Embryo |
| Model | Knock-Out |
| Relevance | The MEF OGG1 KO cell line can be used to assess the role of the mammalian enzyme OGG1 in repair of DNA damage and prevention of carcinogenesis. the cell line can be used to assess the incidence and consequences of 8-oxoG formation in the mammalian genome. |
| Research Area | Cancer, DNA Damage and Repair, Drug Discovery & Development |
| Recommended Growing Conditions | DMEM/Ham’s F-12 (3:1) with 10% FBS |
| Notes | Primary mouse embryo fibroblast (MEF) cultures from Ogg1 mutant mice were established by standard procedures from individual embryonic day 13.5 (E13.5) embryos derived from heterozygous matings. Cells were cultured in DMEM/Ham’s F-12 (3:1) with 10% FBS and genotyped by Southern hybridization, and permanent cell lines were established from transformed clones arising spontaneously after repeated passage in culture. |
| Cellosaurus ID | CVCL_6E74 |
References: 2 entries
Klungland et al. 1999. Proc Natl Acad Sci U S A. 96(23):13300-5. PMID: 10557315.
Accumulation of premutagenic DNA lesions in mice defective in removal of oxidative base damage.
Europe PMC ID: 10557315
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References: 2 entries
Klungland et al. 1999. Proc Natl Acad Sci U S A. 96(23):13300-5. PMID: 10557315.
Accumulation of premutagenic DNA lesions in mice defective in removal of oxidative base damage.
Add a reference
