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MEF OGG1 KO Cell Line

Info

Catalogue Number 151846
Antigen/Gene or Protein Targets OGG1
Host Mouse
Tissue Embryo
Model Knock-Out
Relevance The MEF OGG1 KO cell line can be used to assess the role of the mammalian enzyme OGG1 in repair of DNA damage and prevention of carcinogenesis. the cell line can be used to assess the incidence and consequences of 8-oxoG formation in the mammalian genome.
Research Area Cancer, DNA Damage and Repair, Drug Discovery & Development
Recommended Growing Conditions DMEM/Ham’s F-12 (3:1) with 10% FBS
Notes Primary mouse embryo fibroblast (MEF) cultures from Ogg1 mutant mice were established by standard procedures from individual embryonic day 13.5 (E13.5) embryos derived from heterozygous matings. Cells were cultured in DMEM/Ham’s F-12 (3:1) with 10% FBS and genotyped by Southern hybridization, and permanent cell lines were established from transformed clones arising spontaneously after repeated passage in culture.
Cellosaurus ID CVCL_6E74

References: 1 entry

Klungland et al. 1999. Proc Natl Acad Sci U S A. 96(23):13300-5. PMID: 10557315.


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References: 1 entry

Klungland et al. 1999. Proc Natl Acad Sci U S A. 96(23):13300-5. PMID: 10557315.


Add a reference

References: 1 entry

Klungland et al. 1999. Proc Natl Acad Sci U S A. 96(23):13300-5. PMID: 10557315.


Add a reference


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