Anti-TIMP2 [3A4]
Invented by Ayham Alnabulsi from Vertebrate Antibodies Limited
Invented at Vertebrate Antibodies Limited
- Datasheet
- References (10)
- Inventor Info
Info
![Image for Anti-TIMP2 [3A4]](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,q_auto/10ac0825-19b1-4a2f-9f60-944891bd9556.jpg)
![Image for Anti-TIMP2 [3A4]](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,q_auto/3c8a6d86-a676-4b4d-8711-9350683a99a2.jpg)
![Image for Anti-TIMP2 [3A4]](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,q_auto/a3405792-598c-4e0c-8eaa-2b6056c0f868.jpg)
![Immunohistochemistry image showing positive staining of Anti-TIMP2 [3A4] in Gilthead Sea Bream (Sparus aurata). BLAST analysis has shown that peptide immunogen used to develop Anti-TIMP2 [3A4] is 100% identical in most vertebrates including fish. Immunohistochemistry performed on fixed spleen of Gilthead Sea Bream (Sparus aurata) is showing a positive staining in granulocytes and lymphocytes residing in blood vessels. Erythrocytes are negative.](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,q_auto/d1c4474d-fd3d-4b65-a09b-b2513a1cca5b.png)
![IHC performed on formalin fixed paraffin-embedded sections of normal human pancreas tissue probed with anti-TIMP2 antibody [3A4]. Positive staining is observed in the Islets of Langerhan.](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,q_auto/bcf6863b-60e1-4a88-a51d-8b470cc7b622.jpg)
![IHC performed on formalin fixed paraffin-embedded sections of breast cancer tissue probed with anti-TIMP2 antibody [3A4].](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,q_auto/327ab2ad-8274-4d2d-9d13-24529806cc9e.jpg)
![IHC performed on formalin fixed paraffin-embedded sections of colon cancer tissue probed with anti-TIMP2 antibody [3A4].](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,q_auto/a61ab7f3-a497-49fe-818c-4791ddc02a22.jpg)
![IHC performed on formalin fixed paraffin-embedded sections of hepatocellular carcinoma probed with anti-TIMP2 antibody [3A4].](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,q_auto/c6fe6e52-f64e-451b-9e77-71be789e177f.jpg)
![IHC performed on formalin fixed paraffin-embedded sections of lung cancer tissue probed with anti-TIMP2 antibody [3A4].](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,q_auto/fda4726e-8346-47a9-a070-88cdcb768715.jpg)
![Image for Anti-TIMP2 [3A4]](https://res.cloudinary.com/ximbio/image/upload/c_limit,fl_lossy,h_282,q_auto,w_368/10ac0825-19b1-4a2f-9f60-944891bd9556.jpg)
![Image for Anti-TIMP2 [3A4]](https://res.cloudinary.com/ximbio/image/upload/c_limit,fl_lossy,h_282,q_auto,w_368/3c8a6d86-a676-4b4d-8711-9350683a99a2.jpg)
![Image for Anti-TIMP2 [3A4]](https://res.cloudinary.com/ximbio/image/upload/c_limit,fl_lossy,h_282,q_auto,w_368/a3405792-598c-4e0c-8eaa-2b6056c0f868.jpg)
![Immunohistochemistry image showing positive staining of Anti-TIMP2 [3A4] in Gilthead Sea Bream (Sparus aurata). BLAST analysis has shown that peptide immunogen used to develop Anti-TIMP2 [3A4] is 100% identical in most vertebrates including fish. Immunohistochemistry performed on fixed spleen of Gilthead Sea Bream (Sparus aurata) is showing a positive staining in granulocytes and lymphocytes residing in blood vessels. Erythrocytes are negative.](https://res.cloudinary.com/ximbio/image/upload/c_limit,fl_lossy,h_282,q_auto,w_368/d1c4474d-fd3d-4b65-a09b-b2513a1cca5b.png)
![IHC performed on formalin fixed paraffin-embedded sections of normal human pancreas tissue probed with anti-TIMP2 antibody [3A4]. Positive staining is observed in the Islets of Langerhan.](https://res.cloudinary.com/ximbio/image/upload/c_limit,fl_lossy,h_282,q_auto,w_368/bcf6863b-60e1-4a88-a51d-8b470cc7b622.jpg)
![IHC performed on formalin fixed paraffin-embedded sections of breast cancer tissue probed with anti-TIMP2 antibody [3A4].](https://res.cloudinary.com/ximbio/image/upload/c_limit,fl_lossy,h_282,q_auto,w_368/327ab2ad-8274-4d2d-9d13-24529806cc9e.jpg)
![IHC performed on formalin fixed paraffin-embedded sections of colon cancer tissue probed with anti-TIMP2 antibody [3A4].](https://res.cloudinary.com/ximbio/image/upload/c_limit,fl_lossy,h_282,q_auto,w_368/a61ab7f3-a497-49fe-818c-4791ddc02a22.jpg)
![IHC performed on formalin fixed paraffin-embedded sections of hepatocellular carcinoma probed with anti-TIMP2 antibody [3A4].](https://res.cloudinary.com/ximbio/image/upload/c_limit,fl_lossy,h_282,q_auto,w_368/c6fe6e52-f64e-451b-9e77-71be789e177f.jpg)
![IHC performed on formalin fixed paraffin-embedded sections of lung cancer tissue probed with anti-TIMP2 antibody [3A4].](https://res.cloudinary.com/ximbio/image/upload/c_limit,fl_lossy,h_282,q_auto,w_368/fda4726e-8346-47a9-a070-88cdcb768715.jpg)
![Image thumbnail for Anti-TIMP2 [3A4]](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,h_45,q_auto/10ac0825-19b1-4a2f-9f60-944891bd9556.jpg)
![Image thumbnail for Anti-TIMP2 [3A4]](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,h_45,q_auto/3c8a6d86-a676-4b4d-8711-9350683a99a2.jpg)
![Image thumbnail for Anti-TIMP2 [3A4]](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,h_45,q_auto/a3405792-598c-4e0c-8eaa-2b6056c0f868.jpg)
![Image thumbnail for Anti-TIMP2 [3A4]](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,h_45,q_auto/58ebf2d3-3bf1-4e3c-a4a2-f1b3c9dadd85.png)
![Image thumbnail for Anti-TIMP2 [3A4]](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,h_45,q_auto/d1c4474d-fd3d-4b65-a09b-b2513a1cca5b.png)
![Image thumbnail for Anti-TIMP2 [3A4]](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,h_45,q_auto/bcf6863b-60e1-4a88-a51d-8b470cc7b622.jpg)
![Image thumbnail for Anti-TIMP2 [3A4]](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,h_45,q_auto/327ab2ad-8274-4d2d-9d13-24529806cc9e.jpg)
![Image thumbnail for Anti-TIMP2 [3A4]](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,h_45,q_auto/a61ab7f3-a497-49fe-818c-4791ddc02a22.jpg)
![Image thumbnail for Anti-TIMP2 [3A4]](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,h_45,q_auto/c6fe6e52-f64e-451b-9e77-71be789e177f.jpg)
![Image thumbnail for Anti-TIMP2 [3A4]](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,h_45,q_auto/fda4726e-8346-47a9-a070-88cdcb768715.jpg)
| Catalogue Number | 151837 |
| Applications | ELISA IHC WB |
| Antigen/Gene or Protein Targets | TIMP2 (tissue inhibitor of metalloproteinases 2) |
| Reactivity | Human, Mouse, Rat and Zebrafish |
| Relevance |
Matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix (ECM) in normal physiological processes as well as in disease processes. Tissue inhibitors of metalloproteinases (TIMPs) are the main physiological regulators of the MMPs. The TIMPs are secreted proteins that complex with individual MMPs and regulate the activity of specific MMPs. Together, the MMPs and TIMPs form a complex biological system strictly controlling degradation of ECM. The MMPs and TIMPs have a significant role in facilitating tumour invasion and metastasis. Expression of TIMP2 has been identified in individual studies as prognostic biomarkers in established and locally advanced colorectal cancer. |
| Host | Mouse |
| Immunogen | Ovalbumin-conjugated synthetic peptide; DSGNDIYGNPI |
| Positive Control | IHC: formalin-fixed, paraffin-embedded colonic adenocarcinoma Western blot: rhTIMP-2, 400 ng per lane |
| Subclass | IgG2b |
| Myeloma Used | P3X63Ag8.653 |
| Recommended Growing Conditions | Dulbecco’s media containing L-Glutamine and 20% Fetal Bovine serum (DH20) . |
| Strain | Balb/c |
| Notes | (i) no cross-reactivity with TIMP-1 in immunoblot, (ii) staining in antigen-positive wax-imbedded tissues |
| Research Area | Adhesion, Cancer, Cardiovascular, Cell Signaling & Signal Transduction, Protein Degradation |
References: 10 entries
Keren-Shaul et al. 2017. Cell. 169(7):1276-1290.e17. PMID: 28602351.
IHC
A Unique Microglia Type Associated with Restricting Development of Alzheimer's Disease.
Europe PMC ID: 28602351
Jeffery et al. 2009. Histopathology. 54(7):820-8. PMID: 19635101.
The matrix metalloproteinase/tissue inhibitor of matrix metalloproteinase profile in colorectal polyp cancers.
Europe PMC ID: 19635101
Lyall et al. 2006. Clin Cancer Res. 12(4):1184-91. PMID: 16489072.
Profiling markers of prognosis in colorectal cancer.
Europe PMC ID: 16489072
Curran et al. 2004. Clin Cancer Res. 10(24):8229-34. PMID: 15623598.
Matrix metalloproteinase/tissue inhibitors of matrix metalloproteinase phenotype identifies poor prognosis colorectal cancers.
Europe PMC ID: 15623598
Murray et al. 1998. Gut. 43(6):791-7. PMID: 9824606.
Matrix metalloproteinases and their inhibitors in gastric cancer.
Europe PMC ID: 9824606
Add a reference
References: 10 entries
Keren-Shaul et al. 2017. Cell. 169(7):1276-1290.e17. PMID: 28602351.
IHC
A Unique Microglia Type Associated with Restricting Development of Alzheimer's Disease.
Jeffery et al. 2009. Histopathology. 54(7):820-8. PMID: 19635101.
The matrix metalloproteinase/tissue inhibitor of matrix metalloproteinase profile in colorectal polyp cancers.
Lyall et al. 2006. Clin Cancer Res. 12(4):1184-91. PMID: 16489072.
Profiling markers of prognosis in colorectal cancer.
Curran et al. 2004. Clin Cancer Res. 10(24):8229-34. PMID: 15623598.
Matrix metalloproteinase/tissue inhibitors of matrix metalloproteinase phenotype identifies poor prognosis colorectal cancers.
Murray et al. 1998. Gut. 43(6):791-7. PMID: 9824606.
Matrix metalloproteinases and their inhibitors in gastric cancer.
Add a reference
