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Anti-TIMP1 [2A5]

Invented by Ayham Alnabulsi at Vertebrate Antibodies Limited

Info

Catalogue Number 151836
Applications ELISA IHC WB
Antigen/Gene or Protein Targets TIMP1 (tissue inhibitor of metalloproteinases 1)
Reactivity Human
Relevance Matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix (ECM) in normal physiological processes as well as in disease processes. Tissue inhibitors of metalloproteinases (TIMPs) are the main physiological regulators of the MMPs. The TIMPs are secreted proteins that complex with individual MMPs and regulate the activity of specific MMPs. Together, the MMPs and TIMPs form a complex biological system strictly controlling degradation of ECM. The MMPs and TIMPs have a significant role in facilitating tumour invasion and metastasis.

Expression of TIMP1 has been identified in individual studies as prognostic biomarkers in established and locally advanced colorectal cancer.
Host Mouse
Immunogen Ovalbumin-conjugated synthetic peptide; FQALGDAADIR
Subclass IgG1 kappa
Positive Control IHC: formalin-fixed, paraffin-embedded colonic adenocarcinoma. Western blot: rhTIMP-1, 400 ng per lane
Myeloma Used P3X63Ag8.653
Recommended Growing Conditions Dulbecco’s media containing L-Glutamine and 20% Fetal Bovine serum (DH20).
Strain Balb/c
Notes IHC: A recommended dilution of 1/8. Recommended pretreatment of antigen retrieval: microwave for 7 minutes at 950W in 0.01M sodium citrate buffer, pH 6.0.

Western blot: not reactive with TIMP-2. A recommended dilution of 1/5.
Research Area Adhesion, Cancer, Cardiovascular, Cell Signaling & Signal Transduction, Protein Degradation

References: 4 entries

Jeffery et al. 2009. Histopathology. 54(7):820-8. PMID: 19635101.

IHC

Lyall et al. 2006. Clin Cancer Res. 12(4):1184-91. PMID: 16489072.

Curran et al. 2004. Clin Cancer Res. 10(24):8229-34. PMID: 15623598.

IHC

Murray et al. 1998. Gut. 43(6):791-7. PMID: 9824606.

IHC


Add a reference

References: 4 entries

Jeffery et al. 2009. Histopathology. 54(7):820-8. PMID: 19635101.

IHC

Lyall et al. 2006. Clin Cancer Res. 12(4):1184-91. PMID: 16489072.

Curran et al. 2004. Clin Cancer Res. 10(24):8229-34. PMID: 15623598.

IHC

Murray et al. 1998. Gut. 43(6):791-7. PMID: 9824606.

IHC


Add a reference

References: 4 entries

Jeffery et al. 2009. Histopathology. 54(7):820-8. PMID: 19635101.

IHC

Lyall et al. 2006. Clin Cancer Res. 12(4):1184-91. PMID: 16489072.

Curran et al. 2004. Clin Cancer Res. 10(24):8229-34. PMID: 15623598.

IHC

Murray et al. 1998. Gut. 43(6):791-7. PMID: 9824606.

IHC


Add a reference


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