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Anti-Phospho CHMP4C, Polyclonal [pCHMP4C]

Info

Catalogue Number 151827
Applications WB
Antigen/Gene or Protein Targets Phospho charged multi vesicular protein 4c
Reactivity Mammalian
Relevance Borealin interacts directly with Snf7/Shrb/CHMP4 components in both Drosophila and human cells and the two proteins colocalize at the midbody in late cytokinesis. Aurora B phosphorylates CHMP4C at three serine residues located in its C-terminal linker region, a part of the protein known to regulate its ability to form polymers and interact with the membrane. Over-expression of CHMP4C variants mutated in these three residues caused cytokinesis failure, suggesting that Aurora B inhibits CHMP4C activity during cytokinesis. It is proposed that CPC controls abscission timing in both flies and human cells by regulating the function of ESCRT-III Snf7 proteins during cytokinesis through the interaction of its Borealin component with the N-terminus of Shrb/CHMP4 proteins and Aurora B-mediated phosphorylation of the CHMP4C regulatory linker tail.
Host Rabbit
Immunogen Synthetic peptide (TARRSRAASSQRAEEC)
Molecular Weight (kDa) ~30
Notes Staining Pattern: CHMP4C is predominantly cytoplasmic and partially colocalizes with Borealin during late cytokinesis, and during abscission CHMP4 is found at the midbody.
Positive Control Synchronised HeLa cell extracts. Signals are absent in a twin blot that had been pre-incubated with lambda-phosphatase, indicating that the antibody specifically recognizes a phosphorylated form of CHMP4C.
Research Area Cell Cycle

References: 2 entries

Petsalaki et al. 2016. Nat Commun. 7:11451. PMID: 27126587.

WB

Capalbo et al. 2012. Open Biol. 2(5):120070. PMID: 22724069.


Add a reference

References: 2 entries

Petsalaki et al. 2016. Nat Commun. 7:11451. PMID: 27126587.

WB

Capalbo et al. 2012. Open Biol. 2(5):120070. PMID: 22724069.


Add a reference

References: 2 entries

Petsalaki et al. 2016. Nat Commun. 7:11451. PMID: 27126587.

WB

Capalbo et al. 2012. Open Biol. 2(5):120070. PMID: 22724069.


Add a reference


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