Anti-Phospho CHMP4C, Polyclonal [pCHMP4C]
Invented at University of Cambridge
- Datasheet
- References (4)
- Inventor Info
Info
| Catalogue Number | 151827 |
| Applications | WB |
| Antigen/Gene or Protein Targets | Phospho charged multi vesicular protein 4c |
| Reactivity | Mammalian |
| Relevance | Borealin interacts directly with Snf7/Shrb/CHMP4 components in both Drosophila and human cells and the two proteins colocalize at the midbody in late cytokinesis. Aurora B phosphorylates CHMP4C at three serine residues located in its C-terminal linker region, a part of the protein known to regulate its ability to form polymers and interact with the membrane. Over-expression of CHMP4C variants mutated in these three residues caused cytokinesis failure, suggesting that Aurora B inhibits CHMP4C activity during cytokinesis. It is proposed that CPC controls abscission timing in both flies and human cells by regulating the function of ESCRT-III Snf7 proteins during cytokinesis through the interaction of its Borealin component with the N-terminus of Shrb/CHMP4 proteins and Aurora B-mediated phosphorylation of the CHMP4C regulatory linker tail. |
| Host | Rabbit |
| Immunogen | Synthetic peptide (TARRSRAASSQRAEEC) |
| Molecular Weight (kDa) | ~30 |
| Notes | Staining Pattern: CHMP4C is predominantly cytoplasmic and partially colocalizes with Borealin during late cytokinesis, and during abscission CHMP4 is found at the midbody. |
| Positive Control | Synchronised HeLa cell extracts. Signals are absent in a twin blot that had been pre-incubated with lambda-phosphatase, indicating that the antibody specifically recognizes a phosphorylated form of CHMP4C. |
| Research Area | Cell Cycle |
References: 4 entries
Petsalaki et al. 2016. Nat Commun. 7:11451. PMID: 27126587.
WB
Clks 1, 2 and 4 prevent chromatin breakage by regulating the Aurora B-dependent abscission checkpoint.
Europe PMC ID: 27126587
Capalbo et al. 2012. Open Biol. 2(5):120070. PMID: 22724069.
The chromosomal passenger complex controls the function of endosomal sorting complex required for transport-III Snf7 proteins during cytokinesis.
Europe PMC ID: 22724069
Add a reference
References: 4 entries
Petsalaki et al. 2016. Nat Commun. 7:11451. PMID: 27126587.
WB
Clks 1, 2 and 4 prevent chromatin breakage by regulating the Aurora B-dependent abscission checkpoint.
Capalbo et al. 2012. Open Biol. 2(5):120070. PMID: 22724069.
The chromosomal passenger complex controls the function of endosomal sorting complex required for transport-III Snf7 proteins during cytokinesis.
Add a reference
