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Monoclonal Antibodies

Anti-Integrin aVb6 [7k]

Invented at Queen Mary University of London
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Image thumbnail for Anti-Integrin aVb6 [7k]
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DX3puroB6 (B6) and DX3puro (Pu) cell lysates were tested by western blotting. Blots were stripped and re-probed with the loading control HSC-70. Results with goat anti-B6 (C19) positive control show that B6 appears as a doublet. 3H, 10B2, 62OW, 310W, 12D and 7K also detect a doublet for B6, 59A and 7D detect mostly bands smaller than B6 and 53a.2 detected no bands. The experiments were repeated at least twice for each antibody.
avb6 was immunoprecipitated with rat mAbs and western blotted for B6 with C19 antibody. Film was then exposed for different time exposures. The control (Con) lane was DX3puroB6 lysate. 59A, 10B2, 620W and 12D shows a very good result in revealing the double band for β6 by 5 seconds and 53a2 shows only the upper band of B6. By 1 minute most rat mAbs reveal a B6 immunoprecipitate. Data are from one of three separate experiments.
Representative immunofluorescence results showing the staining of HACAT (left image) and VB6 cells (right image) using anti-Integrin aVb6 [7k].
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Catalogue Number 151818
Applications ELISA FACS IHC IF IP WB
Antigen/Gene or Protein Targets Integrin alpha-v beta6
Reactivity Human
Relevance 7k is selective for AvB6 and particularly useful for detection of AvB6 by immunohistochemisty in paraffin tissue slices. The antibody also recognises AvB6 by FACS and ELISA
Host Rat
Immunogen 3T3b6, mouse fibroblast expressing human beta6
Positive Control HaCaT, BT474
Subclass IgG2a
Myeloma Used Y3.AG.1.2.3
Recommended Growing Conditions DMEM + 10% FCS/ RPMI + 10% FCS
Notes Other Research Ares: Fibrosis

Suggested Dilutions: IHC 1:100 - 1:200 from stock (0.86mg/ml); FACS 20ug/ml; IP 10ul stock (0.86mg/ml) per ml cell lysate (total 100-500ug cellular protein)

Alternate name: MAL/25/4b/7k

Does not bind mouse.
Research Area Adhesion, Cancer, Cardiovascular, Cell Signaling & Signal Transduction, Stem Cell Biology

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Invented at

Queen Mary University of London
Queen Mary University of London

126 reagents listed on Ximbio