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Monoclonal Antibodies

Anti-ASAP1 [7B12]

Invented at Karlsruhe Institute of Technology
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Adapted from Müller et al. 2010. Oncogene; 29(16):2393-403. PMID: 20154719. Figure. Expression of ASAP1 protein in lysates of ASML and 1AS cells. ASAP1 was detected using western blot analysis and the 7B12 antibody. The position of the molecular weight markers is indicated (kDa). The western blot was subsequently stripped and probed with an anti-actin antibody as a loading control.
Adapted from Müller et al. 2010. Oncogene; 29(16):2393-403. PMID: 20154719. Figure. Expression of ASAP1 in human tumors. Normal esophageal, colorectal, and thyroid tissues (a, c, and e, respectively) and their corresponding tumors (b, d, and f, respectively) were immunostained using the 7B12 antibody and AEC (red color), followed by counterstaining with hematoxylin (blue color).
Adapted from Müller et al. 2010. Oncogene; 29(16):2393-403. PMID: 20154719. Figure d. Expression constructs for Flag-tagged ASAP1b or ASAP1c were transfected into HEK293 cells. Lysates were immunoprecipitated in the presence or absence of anti-Flag M2 antibodies, anti-TNP control antibodies, and Protein G–agarose (Resin) as indicated. Immunoprecipitates were western blotted together with total lysate as a positive control. Blots were probed with anti-SLK antibodies (IB SLK), then stripped and probed with 7B12 (IB ASAP1).
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Catalogue Number 151767
Applications ELISA IHC IP WB
Antigen/Gene or Protein Targets ASAP1
Reactivity Human and Rat
Relevance ASAP1 possesses phosphatidylinositol 4,5-biphosphate-dependent GTPase-activating protein activity for ARF1 (ADP ribosylation factor 1) and ARF5 and a lesser activity towards ARF6. It may coordinate membrane trafficking with cell growth or actin cytoskeleton remodeling by binding to both SRC and PIP2. It potentially has involvement in tumour progression, having been shown to promote metastasis formation in vivo and stimulate tumor cell motility, invasiveness, and adhesiveness in vitro. ASAP1 represents a potential target for cancer therapy.
Host Mouse
Immunogen Recombinant Protein (fragment of human ASAP1 (corresponding nucleotides 977-1532 of KIAA1249) with N-terminal GST tag produced in E.coli strain BL-21)
Positive Control Human: HT29 and MDA-MB-231 Rat: ASAP1 is strongly expressed in ASML and weakly expressed in 1AS pancreatic carcinoma cells.
Subclass IgG2b
Myeloma Used Sp2/0-Ag14
Recommended Growing Conditions 10% FCS + RPMI + Pen/Strep
Notes Other research areas applicable: Metastasis, cytoskeleton, vesicle trafficking.

WB WB: Use a concentration of 1 µg/ml. Predicted molecular weight: 125 kDa.

IHC-P IHC-P: Use a concentration of 5 µg/ml.

IHC-Fr IHC-Fr: Use a concentration of 5 µg/ml.

IP IP: Use a concentration of 10 µg/ml.

ELISA ELISA: Use at an assay dependent concentration.
Research Area Cancer, Cell Type or Organelle Marker, Cell Signaling & Signal Transduction

References

There are 2 reference entries for this reagent.

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References: 2 entries

Müller et al. 2010. Oncogene. 29(16):2393-403. PMID: 20154719.

WB IHC

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ASAP1 promotes tumor cell motility and invasiveness, stimulates metastasis formation in vivo, and correlates with poor survival in colorectal cancer patients.

Europe PMC ID: 20154719

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References: 2 entries

Müller et al. 2010. Oncogene. 29(16):2393-403. PMID: 20154719.

WB IHC

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ASAP1 promotes tumor cell motility and invasiveness, stimulates metastasis formation in vivo, and correlates with poor survival in colorectal cancer patients.

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Inventor Information

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Invented at

Karlsruhe Institute of Technology
Karlsruhe Institute of Technology

3 reagents listed on Ximbio