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Anti-Keratin 7 & 8 [LDS68]
RECENTLY UPDATED

Invented by Prof Jenny Southgate from University Of York
Invented at Cancer Research UK London Research Institute: Lincoln's Inn Fields

Info

Catalogue Number 151743
Applications IHC
Antigen/Gene or Protein Targets Keratin 7, 8 in basal, prickle and intermediate cells
Reactivity Human
Relevance Monoclonal antibody directed against closely related keratin's 7 and 8, useful in studying epithelial cells and tumours of epithelial origin.

Background and Research Application
Keratins are a family of intermediate filament proteins that assemble into filaments by forming heterodimers of two different keratin strands - a type I keratin (keratins 9 to 23) and a type II keratin (keratins 1 to 8). Keratins are expressed in specific tissues, with determined differentiation patterns. They are characteristic of epithelial cells and trichocytes.
Keratins 7 and 8 are two closely related type II keratins characteristic of simple epithelia. Keratin 7 is less widespread than keratin 8 and is expressed in sebaceous and sweat glands and some cells of the inner hair root sheath. Keratin 7 is often co-expressed with keratin 19.
The LdS68 antibody is useful for studies of epithelial cells and tumour origin. This antibody reacts specifically with a variety of human simple epithelia (e.g. bile ducts, mammary gland, sweat glands, urinary bladder, endocervix, placenta), of the stomach, colon, pancreatic acini, hepatocytes and myoepithelial cells. It does not react with epidermis or non-epithelial tissues.
Host Mouse
Immunogen Cytoskeletons of the human urothelial RT4 cell line.
Immunogen UniProt ID P08729
Subclass IgG1
Recommended Growing Conditions DMEM + 5% FCS
Notes Production Details
Purified using multi-step affinity chromatography with protein A.

Storage Conditions
Store at -20 degrees frozen. Avoid repeated freeze/thaw cycles.

Points of Interest
This antibody is reactive with methanol or acetone fixed frozen sections, and with certain protease-digested, formalin fixed, paraffin embedded human tissues. Anti-keratin 7&8 reacts specifically with most simple epithelia, but shows no reaction with epithelial cells in the intestine or in the stomach.
Anti-keratin 7&8 had equally positive immunofluorescence reactions for basal, prickle and intermediate oral epithelium layers but was negative for superficial epithelium.

Concentration
1mg/ml as standard
Research Area Cell Signaling & Signal Transduction

References

There are 4 reference entries for this reagent.

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References: 4 entries

Southgate et al. 1987. Lab Invest. 56(2):211-23. PMID: 2433501.

Primary culture of human oral epithelial cells. Growth requirements and expression of differentiated characteristics.

Europe PMC ID: 2433501

Trejdosiewicz et al. 1986. Exp Cell Res. 164(2):388-98. PMID: 2423348.

Phenotypic analysis of cultured melanoma cells. Expression of cytokeratin-type intermediate filaments by the M5 human melanoma cell line.

Europe PMC ID: 2423348


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References: 4 entries

Southgate et al. 1987. Lab Invest. 56(2):211-23. PMID: 2433501.

Primary culture of human oral epithelial cells. Growth requirements and expression of differentiated characteristics.

Trejdosiewicz et al. 1986. Exp Cell Res. 164(2):388-98. PMID: 2423348.

Phenotypic analysis of cultured melanoma cells. Expression of cytokeratin-type intermediate filaments by the M5 human melanoma cell line.


Add a reference