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hTERT-RPE1 mRuby-APC15 Cell Line

Invented by Dr Jonathon Pines at Gurdon Institute

Info

Catalogue Number 151710
Antigen/Gene or Protein Targets APC15
Parental Line hTERT-RPE1
Host Human
Tissue Eye
Model Transgenic
Relevance The hTERT-RPE1 mRuby-APC15 Cell Line is a tet-inducible APC15 reporter cell line. APC15 is a component of the human cell cycle Anaphase promoting complex/cyclosome (APC/C). It is required for progression from metaphase during cell cycle. The uncharacterised open reading frame C11orf51 has been identified in a systematic proteomic analysis of APC/C purified from HeLa cell extracts. Human C11orf51 is conserved in vertebrates and invertebrates and has homology to Schizosaccharomyces pombe APC15 (PM gene ID: 2540655), and Schizosaccharomyces cerevisiae Mnd2 (PM gene ID: 854843).
Production Details APC15 was amplified by PCR from a full length cDNA clone (ORFeome clone 100066673). Tetracycline inducible cell lines expressing APC15 IRES2 mRuby were generated using the FLIP in system and a modified pCDNA5/FRT/TO vector. The parental tetracycline inducible RPE cell line was created by random integration of an FRT site and a Tet repressor gene into hTERT RPE1 cells followed by selection of a clone that showed high levels of gene expression repression in the absence of tetracycline.
Conditional Yes
Conditional Description Tetracycline inducible
Research Area Cell Cycle
Recommended Growing Conditions F12/DMEM (1:1) media supplemented with 10% FCS, sodium bicarbonate, glutamate and penicillin/streptavidin.
Cellosaurus ID CVCL_5F68

References: 1 entry

Mansfeld et al. 2011. Nat Cell Biol. 13(10):1234-43. PMID: 21926987.


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References: 1 entry

Mansfeld et al. 2011. Nat Cell Biol. 13(10):1234-43. PMID: 21926987.


Add a reference

References: 1 entry

Mansfeld et al. 2011. Nat Cell Biol. 13(10):1234-43. PMID: 21926987.


Add a reference


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