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Anti-APC15, Polyclonal [APC15]

Invented by Dr Jonathon Pines at Gurdon Institute

Info

Catalogue Number 151709
Applications IP WB
Antigen/Gene or Protein Targets Human APC15
Reactivity Human
Relevance The uncharacterised open reading frame C11orf51 has been identified in a systematic proteomic analysis of APC/C purified from HeLa cell extracts. Human C11orf51 is conserved in vertebrates and invertebrates and has homology to S. pombe APC15, and S. cerevisiae Mnd2. hAPC15 is previously uncharacterised. It has been shown for the first time that human APC15 is a component of the Anaphase promoting complex/cyclosome (APC/C) which is required for progression from metaphase during cell cycle. Specifically, APC15 drives the turnover of mitotic checkpoint complexes (MCC)-Cdc20 to make the spindle-assembly checkpoint responsive to kinetochore attachment. Depleting APC15 prevents Cyclin B1 ubiquitylation and degradation because MCCs are locked onto the APC/C and cannot be released when all the kinetochores have attached to the spindle.
Host Guinea Pig
Immunogen Full length His-TEVhAPC15 purified from BL21 E.coli
Molecular Weight (kDa) 14
Positive Control Asynchronous human cell lysates are sufficient.
Research Area Cancer, Cell Cycle

References: 1 entry

Mansfeld et al. 2011. Nat Cell Biol. 13(10):1234-43. PMID: 21926987.


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References: 1 entry

Mansfeld et al. 2011. Nat Cell Biol. 13(10):1234-43. PMID: 21926987.


Add a reference

References: 1 entry

Mansfeld et al. 2011. Nat Cell Biol. 13(10):1234-43. PMID: 21926987.


Add a reference


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