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Monoclonal Antibodies

Anti-CLEC9A [8F9]

Invented at Cancer Research UK London Research Institute: Lincoln's Inn Fields
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Adapted from Canavan et al. 2018. JCI Insight; 3(23). PMID: 30518680 Supplementary Figure. (A) Representative images of immunohistochemical staining for CD141 and Clec9A, in synovial tissue from RA and OA patients. Haematoxylin staining was used for counterstaining.
Adapted from Canavan et al. 2018. JCI Insight; 3(23). PMID: 30518680 Supplementary Figure. Immunofluorescence staining for Clec9. IgG control Nuclei were counterstained with DAPI. Original magnification 20x
Adapted from Sancho et al. 2008. J Clin Invest; 118:2098-110. PMID: 18497879 Figure. (A) Human PBMCs were stained with anti–hDNGR-1 (Clec9) or an isotype-matched control antibody (mouse IgG2a) and counterstained for various leukocyte markers. Histograms show DNGR-1 staining on T cells, B cells, monocytes, NK cells, lineage-negative HLA-DR– cells, and lineage-negative HLA-DR+ cells. Numbers indicate the percentage of hDNGR-1+ cells in the latter fraction. (B) PBMCs depicted in A were gated on lineage-negative HLA-DR+ cells. Dot plots show staining with anti–DNGR-1 or isotype-matched control mAbs against various blood DC subset markers. Numbers represent percentage of cells in each quadrant.
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Catalogue Number 151707
Applications FACS IHC IF
Antigen/Gene or Protein Targets Human DNGR1
Reactivity Human
Relevance Recognises human DNGR-1, also known as CLEC9A, which is a highly specific marker of a human DC subset, as well as a receptor able to recognise a preformed signal exposed on necrotic cells.
Host Mouse
Immunogen RBL-2H3 cells expressing human DNGR-1 fused to an HA epitope
Positive Control Mouse IgG2a isotype-matched control mAbs
Subclass IgG2a
Myeloma Used Sp2/0-Ag14
Recommended Growing Conditions RPMI + 10% FCS + Pen + Strep + Glutamine + 2-ME
Strain Balb/c
Notes Block Fc receptor prior to staining to reduce background.
Research Area Immunology, Stem Cell Biology

References

There are 4 reference entries for this reagent.

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References: 4 entries

Poulin et al. 2010. J Exp Med. 207:1261-71. [PMID: 20479117]

Sancho et al. 2008. J Clin Invest 118:2098-110 .[PMID: 18497879]

Sancho et al. 2009. Nature. 458:899-903. [PMID: 19219027]

Canavan et al. 2018. JCI Insight. 3(23):. PMID: 30518680.

FACS IHC

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References: 4 entries

Poulin et al. 2010. J Exp Med. 207:1261-71. [PMID: 20479117]

Sancho et al. 2008. J Clin Invest 118:2098-110 .[PMID: 18497879]

Sancho et al. 2009. Nature. 458:899-903. [PMID: 19219027]

Canavan et al. 2018. JCI Insight. 3(23):. PMID: 30518680.

FACS IHC

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Inventor Information