Cancer Research Technology
Log in Register
Menu
Search
Monoclonal Antibodies

Anti-CLEC9A [7H11]

Invented at Cancer Research UK London Research Institute: Lincoln's Inn Fields
  • Datasheet
  • Inventor Info
Print

Info

Image thumbnail for Anti-CLEC9A [7H11]
Image thumbnail for Anti-CLEC9A [7H11]
Image thumbnail for Anti-CLEC9A [7H11]
Add an image
Sancho D, Mourão-Sá D, Joffre OP, Schulz O, Rogers NC, Pennington DJ, Carlyle JR, Reis e Sousa C. Tumor therapy in mice via antigen targeting to a novel, DC-restricted C-type lectin. J Clin Invest. 2008 Jun;118(6):2098-110. doi: 10.1172/JCI34584. PMID: 18497879; PMCID: PMC2391066.Biochemical characterization of mouse DNGR-1 protein. (A) Lysate from Phoenix cells stably transfected with a retroviral vector encoding HA-tagged mDNGR-1 was Western blotted (WB) with anti-HA before or after immunoprecipitation with anti-HA or the indicated anti–mDNGR-1 antibodies. (B) Western blot using anti–DNGR-1 mAb 397 to probe lysates of Phoenix cells expressing (+) or not expressing (–) DNGR-1 under reducing (right) and nonreducing (left) conditions.
Adapted from Joffre OP, Sancho D, Zelenay S, Keller AM, Reis e Sousa C. Efficient and versatile manipulation of the peripheral CD4+ T-cell compartment by antigen targeting to DNGR-1/CLEC9A. Eur J Immunol. 2010 May;40(5):1255-65. doi: 10.1002/eji.201040419. PMID: 20333625; PMCID: PMC3064981. Antigen targeting to DNGR-1 leads to MHC class II presentation by CD8α+ DC. (A) B6 mice were injected i.v. with OVA323–339-coupled anti-DNGR-1 mAb or with a similarly conjugated Ab control. Four hours later, CD11c− or CD11c+ splenocytes were isolated and cultured with CFSE-labeled OT-II cells. OT-II proliferation was assessed by flow cytometry. Representative CFSE profiles are shown in the left panel, whereas the absolute number of divided OT-II cells is represented in the right panel. (B) As in (A) but the CD11c+ population was further subdivided into CD8α− and CD8α+ fractions. The left panel shows OT-II proliferation induced by CD8α+ and CD8α− DC following targeting with anti-DNGR-1 mAb, whereas the right panel shows absolute number of OT-II cells obtained after culture with CD8α+ DC isolated from mice injected with PBS, anti-DNGR-1 or control mAb conjugates. (C) B6 mice were challenged with OVA323–339-coupled anti-DNGR-1 mAb. At the indicated time points after injection, antigen presentation by CD8α− (right panel) and CD8α+ (left and right panel) CD11c+ cells was determined as described in (A). For each condition, APC were (A) isolated from two mice and tested separately or (B, C) pooled from two mice and tested in duplicate. Data are the average of duplicate tests and are representative of (A, B) three or (C) two independent experiments.
Adapted from Li J, Ahmet F, Sullivan LC, Brooks AG, Kent SJ, De Rose R, Salazar AM, Reis e Sousa C, Shortman K, Lahoud MH, Heath WR, Caminschi I. Antibodies targeting Clec9A promote strong humoral immunity without adjuvant in mice and non-human primates. Eur J Immunol. 2015 Mar;45(3):854-64. doi: 10.1002/eji.201445127. Epub 2015 Jan 14. PMID: 25487143. Variable antibody responses are induced by targeting Clec9A. Five B6 mice per group were injected i.v. with 0.5 μg isotype control Ab (GL117, GL113) or anti‐Clec9A mAb (10B4, 397, 42D2, 7H11, 1F6) in the presence or absence of poly I:C (50 μg). Plasma samples were taken (A) 2 and (B) 4 weeks postinjection and the anti‐rat reactivity measured by ELISA. Experiments were performed twice and pooled data are presented. Only one group of mice was injected with 0.5 μg GL117± poly I:C, but, similar results were obtained using higher of doses GL117 (not shown). Each point represents the end point titer of one individual mouse and the inserted line represents the geometric mean. Statistical analysis was performed on the log‐transformed data using unpaired two‐tailed t‐test and Welch correction.
Add an image
Image thumbnail for Anti-CLEC9A [7H11] Image thumbnail for Anti-CLEC9A [7H11] Image thumbnail for Anti-CLEC9A [7H11]
Catalogue Number 151706
Applications ELISA FACS IF IP WB
Antigen/Gene or Protein Targets Mouse DNGR1
Synonyms C-Type Lectin Domain Family 9 Member A; HEEE9341; UNQ9341; DNGR-1; CD370; DNGR1
Reactivity Mouse
Relevance Recognises mouse DNGR-1, also known as CLEC9A, which is a highly specific marker of the CD8a+ and the CD103+ DC subsets, as well as a receptor able to recognise a preformed signal exposed on necrotic cells.
Host Rat
Immunogen RBL-2H3 cells expressing mouse DNGR-1 fused to an HA epitope
Positive Control rat IgG1 isotype-matched control mAbs
Subclass IgG1
Myeloma Used Y3.AG.1.2.3
Recommended Growing Conditions RPMI + 10% FCS + Pen + Strep + Glutamine + 2-ME
Notes Other Applications: In Vitro and In Vivo Labelling, tetramer staining, CTL priming In Vivo, Coupling of anti-DNGR-1 to antigens and antigen targeting.
Research Area Immunology, Stem Cell Biology

References

There are 4 reference entries for this reagent.

View All References

References: 4 entries

Joffre et al. 2010. Eur J Immunol. 40:1255-65. [PMID: 20333625]

Sancho et al. 2008. J Clin Invest 118:2098-110 .[PMID: 18497879]

Sancho et al. 2009. Nature. 458:899-903. [PMID: 19219027]

Li et al. 2015. Eur J Immunol. 45(3):854-64. PMID: 25487143.

View  

Add a reference

References: 4 entries

Joffre et al. 2010. Eur J Immunol. 40:1255-65. [PMID: 20333625]

Sancho et al. 2008. J Clin Invest 118:2098-110 .[PMID: 18497879]

Sancho et al. 2009. Nature. 458:899-903. [PMID: 19219027]

Li et al. 2015. Eur J Immunol. 45(3):854-64. PMID: 25487143.

View  

Add a reference

Inventor Information