Anti-CLEC9A [1F6]
Invented at Cancer Research UK London Research Institute: Lincoln's Inn Fields
- Datasheet
- References (1)
- Inventor Info
Info
| Catalogue Number | 151705 |
| Applications | FACS IF WB |
| Antigen/Gene or Protein Targets | Mouse DNGR1 |
| Synonyms | C-Type Lectin Domain Family 9 Member A; HEEE9341; UNQ9341; DNGR-1; CD370; DNGR1 |
| Reactivity | Mouse |
| Relevance |
Monoclonal antibody directed against DNGR-1 marker for CD8+ dendritic cells, which can be exploited for tumour therapy, whilst also recognising necrotic cells. Background and Research Application This antibody recognises mouse DNGR-1, also known as CLEC9A, which is a highly specific marker of the CD8a+ and the CD103+ DC subsets, as well as a receptor able to recognise a preformed signal exposed on necrotic cells. DNGR-1 was found to be expressed in mice at high levels by CD8+ DCs and at low levels by plasmacytoid DCs, but not by other hematopoietic cells. DNGR-1 is a novel, highly specific marker of mouse and human DC subsets that can be exploited for CTL cross-priming and tumour therapy. DNGR-1 ligation is required for effective cross-presentation of dead-cell-associated antigen by recruitment and activation of the tyrosine kinase Syk. DNGR-1 recognizes filamentous actin in association with particular actin-binding domains of cytoskeletal proteins, including spectrin, exposed when cell membranes are damaged. This antibody can recognise both the short and long isoform of DNGR-1, which vary in a segment of the stalk region. |
| Host | Rat |
| Immunogen | RBL-2H3 cells expressing mouse DNGR-1 fused to an HA epitope |
| Immunogen UniProt ID | Q8BRU4 |
| Positive Control | Mouse IgG1 isotype-matched control mAbs |
| Subclass | IgG1 |
| Subclass Notes | Previously stated as rat monoclonal but positive for mouse IgG1 |
| Myeloma Used | Y3.AG.1.2.3 |
| Recommended Growing Conditions | RPMI + 10% FCS + Pen + Strep + Glutamine + 2-ME |
| Notes |
Production Details Purified using multi-step affinity chromatography with protein A. Storage Conditions Store at -20 degrees frozen. Avoid repeated freeze/thaw cycles. Points of Interest Other Applications: In Vitro and In Vivo Labelling, tetramer staining, CTL priming In Vivo, Coupling of anti-DNGR-1 to antigens and antigen targeting. Concentration 1mg/ml as standard |
| Research Area | Immunology, Stem Cell Biology |
