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- Inventor Info
|Applications||FACS IF WB|
|Antigen/Gene or Protein Targets||Mouse DNGR1|
|Synonyms||C-Type Lectin Domain Family 9 Member A; HEEE9341; UNQ9341; DNGR-1; CD370; DNGR1|
Monoclonal antibody directed against DNGR-1 marker for CD8+ dendritic cells, which can be exploited for tumour therapy, whilst also recognising necrotic cells.
Background and Research Application
This antibody recognises mouse DNGR-1, also known as CLEC9A, which is a highly specific marker of the CD8a+ and the CD103+ DC subsets, as well as a receptor able to recognise a preformed signal exposed on necrotic cells.
DNGR-1 was found to be expressed in mice at high levels by CD8+ DCs and at low levels by plasmacytoid DCs, but not by other hematopoietic cells. DNGR-1 is a novel, highly specific marker of mouse and human DC subsets that can be exploited for CTL cross-priming and tumour therapy. DNGR-1 ligation is required for effective cross-presentation of dead-cell-associated antigen by recruitment and activation of the tyrosine kinase Syk. DNGR-1 recognizes filamentous actin in association with particular actin-binding domains of cytoskeletal proteins, including spectrin, exposed when cell membranes are damaged.
This antibody can recognise both the short and long isoform of DNGR-1, which vary in a segment of the stalk region.
|Immunogen||RBL-2H3 cells expressing mouse DNGR-1 fused to an HA epitope|
|Immunogen UniProt ID||Q8BRU4|
|Positive Control||Mouse IgG1 isotype-matched control mAbs|
|Subclass Notes||Previously stated as rat monoclonal but positive for mouse IgG1|
|Recommended Growing Conditions||RPMI + 10% FCS + Pen + Strep + Glutamine + 2-ME|
Purified using multi-step affinity chromatography with protein A.
Store at -20 degrees frozen. Avoid repeated freeze/thaw cycles.
Points of Interest
Other Applications: In Vitro and In Vivo Labelling, tetramer staining, CTL priming In Vivo, Coupling of anti-DNGR-1 to antigens and antigen targeting.
1mg/ml as standard
|Research Area||Immunology, Stem Cell Biology|
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