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Anti-CLEC9A [1F6]

Info

Catalogue Number 151705
Applications FACS IF WB
Antigen/Gene or Protein Targets Mouse DNGR1
Synonyms C-Type Lectin Domain Family 9 Member A; HEEE9341; UNQ9341; DNGR-1; CD370; DNGR1
Reactivity Mouse
Relevance Monoclonal antibody directed against DNGR-1 marker for CD8+ dendritic cells, which can be exploited for tumour therapy, whilst also recognising necrotic cells.


Background and Research Application
This antibody recognises mouse DNGR-1, also known as CLEC9A, which is a highly specific marker of the CD8a+ and the CD103+ DC subsets, as well as a receptor able to recognise a preformed signal exposed on necrotic cells.
DNGR-1 was found to be expressed in mice at high levels by CD8+ DCs and at low levels by plasmacytoid DCs, but not by other hematopoietic cells. DNGR-1 is a novel, highly specific marker of mouse and human DC subsets that can be exploited for CTL cross-priming and tumour therapy. DNGR-1 ligation is required for effective cross-presentation of dead-cell-associated antigen by recruitment and activation of the tyrosine kinase Syk. DNGR-1 recognizes filamentous actin in association with particular actin-binding domains of cytoskeletal proteins, including spectrin, exposed when cell membranes are damaged.
This antibody can recognise both the short and long isoform of DNGR-1, which vary in a segment of the stalk region.
Host Rat
Immunogen RBL-2H3 cells expressing mouse DNGR-1 fused to an HA epitope
Immunogen UniProt ID Q8BRU4
Positive Control Mouse IgG1 isotype-matched control mAbs
Subclass IgG1
Subclass Notes Previously stated as rat monoclonal but positive for mouse IgG1
Myeloma Used Y3.AG.1.2.3
Recommended Growing Conditions RPMI + 10% FCS + Pen + Strep + Glutamine + 2-ME
Notes Production Details
Purified using multi-step affinity chromatography with protein A.

Storage Conditions
Store at -20 degrees frozen. Avoid repeated freeze/thaw cycles.

Points of Interest
Other Applications: In Vitro and In Vivo Labelling, tetramer staining, CTL priming In Vivo, Coupling of anti-DNGR-1 to antigens and antigen targeting.

Concentration
1mg/ml as standard
Research Area Immunology, Stem Cell Biology

References

There are 7 reference entries for this reagent.

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References: 7 entries

Sancho et al. 2008. J Clin Invest 118:2098-110 .[PMID: 18497879]

Sancho et al. 2009. Nature. 458:899-903. [PMID: 19219027]

Warrier et al. 2019. Biosci Rep. 39(10):. PMID: 31652460.

Wang et al. 2019. Front Immunol. 10:1582. PMID: 31379818.

King et al. 2019. PLoS One. 14(8):e0220986. PMID: 31430333.

Shrestha et al. 2018. Vaccines (Basel). 6(4):. PMID: 30445683.

Krishnaswamy et al. 2018. Front Immunol. 9:2169. PMID: 30319629.


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References: 7 entries

Sancho et al. 2008. J Clin Invest 118:2098-110 .[PMID: 18497879]

Sancho et al. 2009. Nature. 458:899-903. [PMID: 19219027]

Warrier et al. 2019. Biosci Rep. 39(10):. PMID: 31652460.

Wang et al. 2019. Front Immunol. 10:1582. PMID: 31379818.

King et al. 2019. PLoS One. 14(8):e0220986. PMID: 31430333.

Shrestha et al. 2018. Vaccines (Basel). 6(4):. PMID: 30445683.

Krishnaswamy et al. 2018. Front Immunol. 9:2169. PMID: 30319629.


Add a reference

Inventor Information