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Monoclonal Antibodies

Anti-MUC7 [EU-MUC7]

Invented by Prof Dallas Swallow from GEE UCL
Invented at University College London
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Adapted from Saitou et al. 2020. Cell Rep. 33(7):108402. PMID: 33207190. Figure, Gland- and cell-specific expression of salivary proteins. (A) Immunohistochemistry of glandular tissues. The SM and PAR acinar cells are highly enriched for amylase (AMY1), statherin (STATH) and lactoperoxidase (LPO) compared to the SL, consistent with these being markers of serous cells. MUC7 and CRISP3 are expressed by a subset of acinar cells of the SM and SL, with little to no expression in the PAR. MUC5B is highly expressed by the SL mucous acinar cells but not by the acinar cells of the PAR or SM, indicating it to be a marker of SL function. ECAD = E cadherin; NUC = nuclei. Scale bar = 25 µm. (B) MUC7 and amylase are expressed by distinct subtypes of serous acinar cells. The mature SM tissue section was immunolabeled for MUC7 (red), AMY (amylase, green) and E-cadherin (blue). (C) Gel electrophoretic separation of WS and glandular secretions (PAR, SMSL) followed by staining of proteins with Coomassie blue and of glycoproteins with periodic acid Schiff stain (pink bands in upper panel), and probing of transfers with antibodies against MUC7, AMY, BPIFA2, CRISP3, and STATH (lower panel).
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Catalogue Number 151698
Applications ELISA IHC WB
Antigen/Gene or Protein Targets EU-MUC7 (Non glycosylated domains of human mucin 7/salivary mucin MG2)
Synonyms AKA: EU-MUC7 - [4D2-1D7]
Reactivity Human
Relevance Anti-EU-MUC7 clone 4D2-1D7can be used to study saliva and salivary related diseases.
Host Mouse
Immunogen KLH conjugated peptide EGRERDHELRHRRHHHQ located in the N-terminal part of the MUC7 protein within the histatin-like domain.was used to immunise Balb/c female mice.
Subclass IgG1 kappa
Myeloma Used Sp2/0-Ag14
Recommended Growing Conditions IMDM medium containing hypoxanthine, aminopterin, thymidine, 20% FCS and 10% Domadrive.
Strain Balb/c
Notes Myeloma: HPRT-deficient SP2/0

No cross reactivity with other human mucins
Research Area Cell Structure and Motility

References

There are 3 reference entries for this reagent.

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References: 3 entries

Saitou et al. 2020. Cell Rep. 33(7):108402. PMID: 33207190.

IHC WB

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Rousseau et al. 2003. Hybrid Hybridomics. 22(5):293-9. PMID: 14678646.

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New monoclonal antibodies to non-glycosylated domains of the secreted mucins MUC5B and MUC7.

Europe PMC ID: 14678646

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References: 3 entries

Saitou et al. 2020. Cell Rep. 33(7):108402. PMID: 33207190.

IHC WB

View  

Rousseau et al. 2003. Hybrid Hybridomics. 22(5):293-9. PMID: 14678646.

View  

New monoclonal antibodies to non-glycosylated domains of the secreted mucins MUC5B and MUC7.

View  

Add a reference

Inventor Information