MEF PKCe KO Cell Line
Invented at Cancer Research UK London Research Institute: Lincoln's Inn Fields
- Datasheet
- References (5)
- Inventor Info
Info
| Catalogue Number | 151665 |
| Antigen/Gene or Protein Targets | PKC epsilon |
| Host | Mouse |
| Tissue | Embryo |
| Disease Keywords | Cancer, heart disease |
| Model | Knock-Out |
| Relevance | The MEF PKCe KO Cell Line is useful for studying the role of PKCe in various biological processes. Clonal MEFs have been derived from PKCepsilon knock-out embryos and subsequently stably transfected with empty pcDNA3 CMV/IE hygo+. Matched isogenic cell line with PKCe re-expressed is also available. Cells have reduced migratory capacity compared to their PKCe-expressing counterparts. Cells were used to demonstrate a role for PKCe in controlling the traffic of beta1 integrins in motile cells |
| Production Details | MEFs were derived from PKCe knockout embryos. Polyclonal PKCeKO cells were transfected with pcDNA3 CMV/IE hygro+ vector using calcium phosphate. Clonal stable cell lines were selected using limiting dilution. |
| Research Area | Adhesion, Cell Signaling & Signal Transduction, Cell Structure and Motility |
| Recommended Growing Conditions | Cells were selected, and are routinely cultured in DMEM + 10% FCS and 100ug/ml hygromycin at 30degC in a 10% CO2 atmosphere |
| Cellosaurus ID | CVCL_AS81 |
References: 5 entries
Cited by 24 subsequent publications including:
Ivaska, J. et al. 2002. EMBO J. 21:3608-19.PMID: 12110574
Kermorgant, S. et al. 2004. EMBO J. 23: 3721:34. PMID 15385963
Pardo et al. 2006. EMBO J. 25(13):3078-88. PMID: 16810323.
FGF-2 protects small cell lung cancer cells from apoptosis through a complex involving PKCepsilon, B-Raf and S6K2.
Europe PMC ID: 16810323
Add a reference
References: 5 entries
Cited by 24 subsequent publications including:
Ivaska, J. et al. 2002. EMBO J. 21:3608-19.PMID: 12110574
Kermorgant, S. et al. 2004. EMBO J. 23: 3721:34. PMID 15385963
Pardo et al. 2006. EMBO J. 25(13):3078-88. PMID: 16810323.
FGF-2 protects small cell lung cancer cells from apoptosis through a complex involving PKCepsilon, B-Raf and S6K2.
Add a reference
