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MEF PKCe KO KI Cell Line

Info

Catalogue Number 151663
Antigen/Gene or Protein Targets PKC epsilon
Host Mouse
Tissue Embryo
Disease Keywords Cancer, heart disease
Model Transgenic
Relevance MEF PKCe KO KI Cell Line is a clonal MEF line derived from PKCepsilon knock-out embryos subsequently transfected to re-express PKCe through stable transfection with pcDNA3 CMV/IE hygo+ PKCe. Matched isogenic cell line which is null for PKCe is also available. Useful for studying the role of PKCe in various biological processes
Production Details MEFs were derived from PKCe knockout embryos. Polyclonal PKCeKO cells were transfected with pcDNA3 CMV/IE hygro+ PKCe vector using calcium phosphate. Clonal stable cell lines were selected using limiting dilution. The vector was contructed by replacing the promoter region in pCMV hygro+ with a 2.1kb CMV/IE promoter derived from p63d. Mouse cDNA encoding full-length PKCe (3.3kb fragment) was ligated to the modified pcDNA3 CMV/IE hygro+ vector.
Research Area Adhesion, Cell Signaling & Signal Transduction, Cell Structure and Motility
Growth/Phenotype Keywords Normal phenotype
Recommended Growing Conditions Cells were selected, and are routinely cultured in DMEM + 10% FCS and 100ug/ml hygromycin at 30degC in a 10% CO2 atmosphere
Cellosaurus ID CVCL_AS82

References: 4 entries

Cited by 24 subsequent publications including:

Ivaska, J. et al. 2002. EMBO J. 21:3608-19.PMID: 12110574

Kermorgant, S. et al. 2004. EMBO J. 23: 3721:34. PMID 15385963

Pardo et al. 2006. EMBO J. 25(13):3078-88. PMID: 16810323.


Add a reference

References: 4 entries

Cited by 24 subsequent publications including:

Ivaska, J. et al. 2002. EMBO J. 21:3608-19.PMID: 12110574

Kermorgant, S. et al. 2004. EMBO J. 23: 3721:34. PMID 15385963

Pardo et al. 2006. EMBO J. 25(13):3078-88. PMID: 16810323.


Add a reference

References: 4 entries

Cited by 24 subsequent publications including:

Ivaska, J. et al. 2002. EMBO J. 21:3608-19.PMID: 12110574

Kermorgant, S. et al. 2004. EMBO J. 23: 3721:34. PMID 15385963

Pardo et al. 2006. EMBO J. 25(13):3078-88. PMID: 16810323.


Add a reference


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