FLYA-13 Cell Line
Invented by Mary Collins from University College London
Invented at The Institute of Cancer Research
- Datasheet
- References (4)
- Inventor Info
Info
| Catalogue Number | 151655 |
| Parental Line | HT 1080 |
| Host | Human |
| Disease Keywords | Fibrosarcoma |
| Model | Packaging |
| Relevance | The FLYA-13 Cell Line is a packaging cell line enabling production of high-titer, human complement-resistant recombinant retroviruses, with significantly reduced probability of replication-competent retrovirus generation. HT 1080-based packaging cell line enabling production of recombinant retroviral vectors with Moloney murine leukemia virus cores and amphotropic murine leukemia virus envelopes. The vectors demonstrate high resistance to the inhibitory effects of human serum/complement, increasing penetration of the vector, and making the system ideal for in vivo gene transfer. |
| Production Details | For details of production of FLYA13 cell line see Cosset et al. 1995. Journal of Virology. 69:7430-36. PMID: 7494248. |
| Research Area | Gene Expression, Genetic Studies Tools |
| Growth/Phenotype Keywords | Recombinant retroviral production |
| Recommended Growing Conditions | For recommended growth and recombinant retrovirus production conditions see Cosset F et al, Journal of Virology, 1995, v69 pp7430-7436 & Takeuchi Y et al, Journal of Virology, 1994, v68 pp8001-8007 |
| Cellosaurus ID | CVCL_8870 |
References: 4 entries
Cosset et al. 1995. J Virol. 69(12):7430-6. PMID: 7494248.
High-titer packaging cells producing recombinant retroviruses resistant to human serum.
Europe PMC ID: 7494248
Takeuchi et al. 1994. J Virol. 68(12):8001-7. PMID: 7966590.
Type C retrovirus inactivation by human complement is determined by both the viral genome and the producer cell.
Europe PMC ID: 7966590
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References: 4 entries
Cosset et al. 1995. J Virol. 69(12):7430-6. PMID: 7494248.
High-titer packaging cells producing recombinant retroviruses resistant to human serum.
Takeuchi et al. 1994. J Virol. 68(12):8001-7. PMID: 7966590.
Type C retrovirus inactivation by human complement is determined by both the viral genome and the producer cell.
Add a reference
