Anti-RSV F Glycoprotein [5A6]
Invented at University of Newcastle upon Tyne
- Datasheet
- References (6)
- Inventor Info
Info
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| Catalogue Number | 151637 |
| Applications | ELISA IHC IF WB |
| Antigen/Gene or Protein Targets | Respiratory syncytial virus subgroup A G glycoprotein |
| Reactivity | Virus |
| Relevance | RSV5A6 may be used for the detection of the fusion glycoprotein of HRSV of both sub-groups A and B. |
| Host | Mouse |
| Immunogen | hRSV strain A2 infected HeLa cells |
| Positive Control | Human RSV A2 infected HeLa cells |
| Subclass | IgG2a |
| Myeloma Used | P3/NS1/1-Ag4.1 |
| Recommended Growing Conditions | RPMI + 10% FCS + Hepes (25mM) + glutamine (2mM) |
| Notes |
Reactivity: Respiratory Syncytial Virus The antibody (IgG2a) was raised against the A2 strain of virus and shown to react in ELISA against a panel of sub-group A strains isolated in Newcastle upon Tyne between 1966 and 1991, and sub-group B strains isolated between 1966 and 1985 as well as the laboratory A strain A2 and B strain 8/60. Epitope is less defined. As well as ELISA the antibody binds the F (specifically the F1) polypeptide in Western blots. It also binds to the F polypeptide expressed in E. coli but not to any of a set of overlapping fragment of F similarly expressed. |
| Research Area | Virology |
References: 6 entries
Touzelet et al. 2020. Mol Cell Proteomics. 19(5):793-807. PMID: 32075873.
WB
Vanover et al. 2017. Nat Commun. 8(1):667. PMID: 28939853.
IF
Wright et al. 1997. J Pathol. 182(2):238-44. PMID: 9274537.
A monoclonal antibody pool for routine immunohistochemical detection of human respiratory syncytial virus antigens in formalin-fixed, paraffin-embedded tissue.
Europe PMC ID: 9274537
West et al. 1994. J Gen Virol. 75 ( Pt 10):2813-9. PMID: 7931172.
Biological activity, binding site and affinity of monoclonal antibodies to the fusion protein of respiratory syncytial virus.
Europe PMC ID: 7931172
Add a reference
References: 6 entries
Touzelet et al. 2020. Mol Cell Proteomics. 19(5):793-807. PMID: 32075873.
WB
Vanover et al. 2017. Nat Commun. 8(1):667. PMID: 28939853.
IF
Wright et al. 1997. J Pathol. 182(2):238-44. PMID: 9274537.
A monoclonal antibody pool for routine immunohistochemical detection of human respiratory syncytial virus antigens in formalin-fixed, paraffin-embedded tissue.
West et al. 1994. J Gen Virol. 75 ( Pt 10):2813-9. PMID: 7931172.
Biological activity, binding site and affinity of monoclonal antibodies to the fusion protein of respiratory syncytial virus.
Add a reference
