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RIP140 KO Mouse

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Catalogue Number 151633
Antigen/Gene or Protein Targets RIP140
Disease Keywords Infertility and obesity
Relevance RIP140 is a ligand-dependent corepressor for most nuclear receptors, and functions through interaction with their AF2 activation domains.Scientific objective was to elucidate the function of RIP140 is oestrogen receptor signalling but was subsequently extended to include other nuclear receptors. This mouse maybe useful for studying compounds targeting RIP140 in fertility and obesity associated disorders. Cofactor for nuclear receptors.
Production Details Recombinant lambda bacteriophage encompassing an 8.1-kilobase XbaI fragment containing the entire coding region of mouse Nrip1 were isolated from a 129/Sv mouse genomic library. A 3.63-kilobase NsiI fragment containing the entire coding sequence with the exception of the first 27 amino acids was removed and replaced with an internal ribosomal entry site neo cassette from the vector pGT1.8IRESGEOSK. The plasmid DNA was electroporated into 129/Ola embryonic stem embryonic stem cells, and G418-resistant clones were screened for homologous recombination using Southern blot analysis. Of 144 embryonic stem cell clones analyzed, 5 underwent homologous recombination at the Nrip1 locus. Two independent Nrip1-/- clones contributed to the germline and were used to generate Nrip1-null lines.
Conditional No
Growth/Phenotype Keywords Females are infertile and both sexes exhibit a lean phenotype, resistant to high-fat diet-induced obseity and hepatic steatosis.
Mouse Genetic Background/Cross History 29/BL6; Backcrossed onto C57 BL/6 mice .
Zygosity Hetero and homozygous mice are viable
Research Area Cell Signaling & Signal Transduction, Epigenetics & Nuclear Signalling, Genetic Studies Tools

References: 2 entries

Nautiyal et al. 2010. Endocrinology. 151(6):2923-32. PMID: 20308529.

White et al. 2000. Nat Med. 6(12):1368-74. PMID: 11100122.


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References: 2 entries

Nautiyal et al. 2010. Endocrinology. 151(6):2923-32. PMID: 20308529.

White et al. 2000. Nat Med. 6(12):1368-74. PMID: 11100122.


Add a reference

References: 2 entries

Nautiyal et al. 2010. Endocrinology. 151(6):2923-32. PMID: 20308529.

White et al. 2000. Nat Med. 6(12):1368-74. PMID: 11100122.


Add a reference


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