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C-Raf KI D486A Cell Line


Catalogue Number 151631
Antigen/Gene or Protein Targets Raf1 D486A oncogenic mutant
Host Mouse
Tissue Embryo
Disease Keywords Cancer
Model Knock-In
Relevance The C-Raf KI D486A Cell Line is derived from the knock-in mouse model carrying oncogenic Raf-1 D486A; it is useful for in vivo study of oncogenic Raf-1 mutants and Ras signalling.
Production Details Embryos were dissected and cut, washed several times with cold PBS and then incubated at 4°C for 2 hours in 0.25% trypsin. Cells were incubated as per the growth conditions. Primary cells were immortalised and resulting cells grown out by continuous culture.
Research Area Cancer, Cell Signaling & Signal Transduction, Drug Discovery & Development
Recommended Growing Conditions DMEM, 10% FCS, 100U/mol penicillin/streptomycin
Notes See the Raf-1 D486A oncogenic mutant knockin mouse that these cells are produced from.

References: 1 entry

Noble et al. 2008. Mol Cell. 31(6):862-72. PMID: 18922468.

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