A375P beta6-puro Cell Line
Invented at Queen Mary University of London
- Datasheet
- References (2)
- Inventor Info
Info
| Catalogue Number | 151626 |
| Antigen/Gene or Protein Targets | Integrin beta 6 |
| Parental Line | A375P |
| Host | Human |
| Disease Keywords | Melanoma |
| Model | Transgenic |
| Relevance | The A375P beta6-puro Cell Line is a useful model for studying the function and activity of beta 6 integrin when used as a matched pair with the parent A375 line. A375 melanoma cells endogenously express a variety of integrins, but not beta 6. These cell lines have been used to validate the activity and selectivity of AvB6-binding peptides. |
| Production Details | The human melanoma cell line A375P was infected with pBabe retroviruses encoding puromycin resistance or, in addition, cDNA for human integrin beta 6. Cells were selected in puromycin (1.25 ug/mL) followed by magnetic bead sorting, using 10D5 (mouse anti-avh6; Chemicon International), according to the manufacturer’s instructions (Dynal; Invitrogen). The avb6 expression can drift so recipients must check by flow cytometery after they have been in continuous passage, at least once a month. Use the magnetic beads above to re-select when this happens. |
| Research Area | Adhesion, Cancer, Cell Signaling & Signal Transduction, Drug Discovery & Development |
| Growth/Phenotype Keywords | A375P melanoma cells stably expressing integrin beta 6 |
| Recommended Growing Conditions | DMEM + 10%FCS/1.25ug/ml puromycin. Antibotic is not required all the time. |
| Cellosaurus ID | CVCL_5F66 |
References: 2 entries
DiCara et al. 2007. J Biol Chem. 282(13):9657-65. PMID: 17244604.
Structure-function analysis of Arg-Gly-Asp helix motifs in alpha v beta 6 integrin ligands.
Europe PMC ID: 17244604
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References: 2 entries
DiCara et al. 2007. J Biol Chem. 282(13):9657-65. PMID: 17244604.
Structure-function analysis of Arg-Gly-Asp helix motifs in alpha v beta 6 integrin ligands.
Add a reference
