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A375P beta6-puro Cell Line


Catalogue Number 151626
Antigen/Gene or Protein Targets Integrin beta 6
Parental Line A375P
Host Human
Disease Keywords Melanoma
Model Transgenic
Relevance The A375P beta6-puro Cell Line is a useful model for studying the function and activity of beta 6 integrin when used as a matched pair with the parent A375 line. A375 melanoma cells endogenously express a variety of integrins, but not beta 6. These cell lines have been used to validate the activity and selectivity of AvB6-binding peptides.
Production Details The human melanoma cell line A375P was infected with pBabe retroviruses encoding puromycin resistance or, in addition, cDNA for human integrin beta 6. Cells were selected in puromycin (1.25 ug/mL) followed by magnetic bead sorting, using 10D5 (mouse anti-avh6; Chemicon International), according to the manufacturer’s instructions (Dynal; Invitrogen). The avb6 expression can drift so recipients must check by flow cytometery after they have been in continuous passage, at least once a month. Use the magnetic beads above to re-select when this happens.
Research Area Adhesion, Cancer, Cell Signaling & Signal Transduction, Drug Discovery & Development
Growth/Phenotype Keywords A375P melanoma cells stably expressing integrin beta 6
Recommended Growing Conditions DMEM + 10%FCS/1.25ug/ml puromycin. Antibotic is not required all the time.
Cellosaurus ID CVCL_5F66

References: 1 entry

DiCara et al. 2007. J Biol Chem. 282(13):9657-65. PMID: 17244604.

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