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|Applications||IHC IF IP WB|
|Antigen/Gene or Protein Targets||Human matrix metalloproteinase 9 (MMP-9)|
Matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix (ECM) in normal physiological processes as well as in disease processes. Tissue inhibitors of metalloproteinases (TIMPs) are the main physiological regulators of the MMPs. The TIMPs are secreted proteins that complex with individual MMPs and regulate the activity of specific MMPs. Together, the MMPs and TIMPs form a complex biological system strictly controlling degradation of ECM. The MMPs and TIMPs have a significant role in facilitating tumour invasion and metastasis.
Expression of MMP9 has been identified in individual studies as prognostic biomarkers in established and locally advanced colorectal cancer.
|Immunogen||Ovalbumin-conjugated synthetic peptide; KLGLGADVAQVT|
|Positive Control||IHC: formalin-fixed, paraffin-embedded lung containing intra-alveolar macrophage. Western Blot: rhMMP-9, 400 ng per lane|
|Recommended Growing Conditions||Dulbecco’s media containing L-Glutamine and 20% Fetal Bovine serum (DH20) .|
IHC: a recommended dilution of 1/32 and pretreatment of antigen retrieval: microwave for 10 minutes at 950W in 0.01M sodium citrate buffer, pH 6.0.
Western Blot: not reactive with MMP-1, -2, -3. A recommended dilution of 1/5.
|Research Area||Adhesion, Cancer, Cardiovascular, Epigenetics & Nuclear Signalling|
References: 7 entries
Sreekanth et al. 2011. Oncogene. 30(28):3139-52. PMID: 21317920.
Jeffery et al. 2009. Histopathology. 54(7):820-8. PMID: 19635101.
Lyall et al. 2006. Clin Cancer Res. 12(4):1184-91. PMID: 16489072.
Curran et al. 2004. Clin Cancer Res. 10(24):8229-34. PMID: 15623598.
Murray et al. 1998. Gut. 43(6):791-7. PMID: 9824606.
Duncan et al. 1998. Eur J Biochem. 258(1):37-43. PMID: 9851689.
Murray et al. 1998. J Pathol. 185(3):256-61. PMID: 9771478.
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