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Polyclonal Antibodies

Anti-Pinin, Polyclonal [Pinin]

Invented by Prof Neil Perkins from University of Newcastle upon Tyne
Invented at University of Dundee
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Adapted from Bracken CP, Wall SJ, Barré B, Panov KI, Ajuh PM, Perkins ND. Regulation of cyclin D1 RNA stability by SNIP1. Cancer Res. 2008 Sep 15;68(18):7621-8. doi: 10.1158/0008-5472.CAN-08-1217. PMID: 18794151; PMCID: PMC2546513. (A) Purification of SNIP1 associated proteins. 10μg of expression plasmids encoding GST-SNIP1 or GST alone were transfected into HEK 293 cells and extracts were prepared prior to purification using glutathione agarose, SDS-PAGE and colloidal coomassie staining. Bands specifically present in the SNIP1-GST lane were excised and proteins identified by MALDI-Tof-Tof (MS/MS). (B) SNIP1 associated proteins are all in a high molecular weight complex of similar size. HeLa nuclear extract was resolved by Superose 6 gel filtration and fractions separated by SDS-PAGE and western blotted using the antibodies indicated. The positions of molecular weight markers used during column calibration are shown. (C) Confirmation of SNIP1 associated proteins by western blot analysis. 293 cells were transfected with THRAP3, SNIP1 or SkIP-HA expression vectors and anti-HA immunoprecipitations performed prior to SDS-PAGE and western blotting for endogenous proteins as indicated. (D) Co-immunoprecipitation of endogenous SNIP1 associated proteins. 200μg of U-2 OS extract was analysed by immunoprecipitation, SDS-PAGE and western blotting using the indicated antibodies. Inputs represent 5% of total protein used in the assay.
Adapted from Carvalho T, Martins S, Rino J, Marinho S, Carmo-Fonseca M. Pharmacological inhibition of the spliceosome subunit SF3b triggers exon junction complex-independent nonsense-mediated decay. J Cell Sci. 2017 May 1;130(9):1519-1531. doi: 10.1242/jcs.202200. Epub 2017 Mar 16. PMID: 28302904. Fluorescence intensities were plotted in a graph as a function of distance (in pixels) to the center of the transcription site. Intensity values corresponding to immunofluorescence (IF) signal are depicted in color, and values corresponding to the DNA FISH signal are depicted in gray. (B–I) The radial analysis was carried out in doxycycline-induced U2OS-βWT cells that were either left untreated (green) or treated with SSA for 6 h (magenta), and in U2OS-βIVS1 cells (blue), using the indicated antibodies. The total number of cells analyzed is indicated in brackets. The graphs depict mean±s.e.m.
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Catalogue Number 151617
Applications ELISA IHC IF IP WB
Antigen/Gene or Protein Targets Pinin
Reactivity Human
Relevance Pinin is a cell adhesion-associated and nuclear protein that has been shown to localize in the vicinity of intermediate filament (IF) convergence upon the cytoplasmic face of the desmosomal plaque as well as in the nucleus. Some data suggest that pinin may play a role in epithelial cell adhesion and the IF complex through a direct interaction with the keratin filaments. Pinin has also been reported to have a role in RNA processing and transcriptional regulation and is part of the SNARP complex. Together with SNIP1 and other proteins Pinin has a role in regulation of proliferation and cancer.
Host Rabbit
Immunogen Recombinant his-tagged protein
Positive Control U2OS cells
Research Area Cancer, Epigenetics & Nuclear Signalling

References

There are 4 reference entries for this reagent.

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References: 4 entries

Carvalho et al. 2017. J Cell Sci. 130(9):1519-1531. PMID: 28302904.

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Bracken et al. 2008. Cancer Res. 68(18):7621-8. PMID: 18794151.

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Bracken et al. 2008. Cancer Res. 68(18):7621-8. PMID: 18794151.

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Regulation of cyclin D1 RNA stability by SNIP1.

Europe PMC ID: 18794151

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References: 4 entries

Carvalho et al. 2017. J Cell Sci. 130(9):1519-1531. PMID: 28302904.

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Bracken et al. 2008. Cancer Res. 68(18):7621-8. PMID: 18794151.

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Bracken et al. 2008. Cancer Res. 68(18):7621-8. PMID: 18794151.

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Regulation of cyclin D1 RNA stability by SNIP1.

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Inventor Information