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Monoclonal Antibodies

Anti-SIGLEC8 [7C9]

Invented by Prof Paul Crocker from University of Dundee
Invented at University of Dundee
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Adapted from Floyd H, Ni J, Cornish AL, Zeng Z, Liu D, Carter KC, Steel J, Crocker PR. Siglec-8. A novel eosinophil-specific member of the immunoglobulin superfamily. J Biol Chem. 2000 Jan 14;275(2):861-6. doi: 10.1074/jbc.275.2.861. PMID: 10625619.Expression of siglec-8 on purified eosinophils. A, eosinophils were purified from granulocytes by negative selection using CD16 immunobeads and analyzed for staining with the anti-siglec-8 mAb, 7C9, or or anti-siglec-5 mAb, 1A5. Eosinophils express siglec-8 uniformly but are negative for siglec-5, which gave a staining pattern identical to that obtained with mouse IgG used as a negative control (not shown). B, micrographs from hematoxylin and eosin-stained preparations of total blood leukocytes (left), CD16-selected neutrophils (center), and purified eosinophils (right).Arrows depict eosinophils before and after purification.
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Catalogue Number 151590
Applications ELISA FACS IP
Antigen/Gene or Protein Targets SIGLEC8
Reactivity Human
Relevance SIGLEC8 is a putative adhesion molecule that mediates sialic-acid dependent binding to cells. It preferentially binds to alpha2,3-linked sialic acid. and also binds to alpha2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. SIGLEC8 is expressed specifically on eosinophils. The protein contains 1 copy of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in downmodulation of cellular responses. The phosphorylated ITIM motif binds to the SH2 domain of PTPN6/SHP-1. The SIGLEC8 gene belongs to the immunoglobulin superfamily.
Host Mouse
Immunogen Siglec-8-Fc protein, containing entire extracellular region of Human Siglec-8 fused with the Fc region of human IgG1
Positive Control Siglec-8 CHO cells vs non-transfected CHO cells
Subclass IgG1
Myeloma Used Sp2/0-Ag14
Recommended Growing Conditions DMEM + 10% Fetal Clone I + 1% penecillin/streptomycin
Strain Balb/c
Notes Recommended dilution for FACS: Cells (1x10^6) should be incubated with 1ug of antibody/100 ul in 1% BSA in PBS for 30–60 min on ice (Nguyen et al., 2006).
Research Area Adhesion, Cell Type or Organelle Marker, Cell Signaling & Signal Transduction, Immunology

References

There are 4 reference entries for this reagent.

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References: 4 entries

Nguyen et al. 2006. Proc Natl Acad Sci U S A. 103(20):7765-70. PMID: 16682635.

FACS

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Loss of Siglec expression on T lymphocytes during human evolution.

Europe PMC ID: 16682635

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Floyd et al. 2000. J Biol Chem. 275(2):861-6. PMID: 10625619.

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Siglec-8. A novel eosinophil-specific member of the immunoglobulin superfamily.

Europe PMC ID: 10625619

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References: 4 entries

Nguyen et al. 2006. Proc Natl Acad Sci U S A. 103(20):7765-70. PMID: 16682635.

FACS

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Loss of Siglec expression on T lymphocytes during human evolution.

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Floyd et al. 2000. J Biol Chem. 275(2):861-6. PMID: 10625619.

View  

Siglec-8. A novel eosinophil-specific member of the immunoglobulin superfamily.

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