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Anti-SIGLEC5 [1A5]

Invented by Prof Paul Crocker at University of Dundee

Info

Catalogue Number 151589
Applications ELISA FACS IHC IF IP Fn WB
Antigen/Gene or Protein Targets Siglec5
Synonyms Sialic Acid Binding Ig Like Lectin 5; Obesity-Binding Protein 2; OB-Binding Protein 2; CD33 Antigen-Like 2; SIGLEC-5; CD33L2; OBBP2; Sialic Acid-Binding Immunoglobulin-Like Lectin 5; CD170 Antigen;
Reactivity Human
Relevance The Siglecs are a family of membrane bound lectins (of the immunoglobulin superfamily) that bind sialic acid and mediate cell-cell interactions. Siglec5 (CD170) is a member of the recently-described human CD33-related siglec subgroup of sialic acid binding Ig-like lectins and is expressed on myeloid cells of the hemopoietic system. Similar to other CD33- related siglecs, Siglec-5 contains two tyrosine based motifs in its cytoplasmic tails implicated in signalling functions. The antibody cross reacts with Siglec14.
Host Mouse
Immunogen Siglec-5-Fc protein, containing entire extracellular region of siglec-5 fused with the Fc region of human IgG1
Subclass IgG1
Positive Control Siglec5 transfected CHO cells. Apart from Siglec14 (Angata T et al. 2006 FASEB J. 20:1964-73. PMID: 17012248), non-cross-reactive with panel of other Siglec proteins, either expressed on CHO cells or as recombinant proteins.
Myeloma Used Sp2/0-Ag14
Recommended Growing Conditions DMEM + 10% FetalClone I + 1% penicillin/streptomycin
Notes Recommended dilution for FACS: Cells (1x10^6) should be incubated with 1ug of antibody/100 ul in 1% BSA in PBS for 30–60 min on ice (Nguyen et al., 2006).
Research Area Adhesion, Cell Type or Organelle Marker, Immunology

References: 11 entries

Angata et al. 2006. FASEB J. 20(12):1964-73. PMID: 17012248.

Discovery of Siglec-14, a novel sialic acid receptor undergoing concerted evolution with Siglec-5 in primates.

Europe PMC ID: 17012248

Nguyen et al. 2006. Proc Natl Acad Sci U S A. 103(20):7765-70. PMID: 16682635.

Nguyen et al. 2006. Proc Natl Acad Sci U S A. 103(20):7765-70. PMID: 16682635.

FACS

Loss of Siglec expression on T lymphocytes during human evolution.

Europe PMC ID: 16682635

Avril et al. 2005. J Biol Chem. 280(20):19843-51. PMID: 15769739.

Siglec-5 (CD170) can mediate inhibitory signaling in the absence of immunoreceptor tyrosine-based inhibitory motif phosphorylation.

Europe PMC ID: 15769739

Connolly et al. 2002. Br J Haematol. 119(1):221-38. PMID: 12358929.

IHC

Human Siglec-5: tissue distribution, novel isoforms and domain specificities for sialic acid-dependent ligand interactions.

Europe PMC ID: 12358929

Cornish et al. 1998. Blood. 92(6):2123-32. PMID: 9731071.

Characterization of siglec-5, a novel glycoprotein expressed on myeloid cells related to CD33.

Europe PMC ID: 9731071


Add a reference

References: 11 entries

Angata et al. 2006. FASEB J. 20(12):1964-73. PMID: 17012248.

Discovery of Siglec-14, a novel sialic acid receptor undergoing concerted evolution with Siglec-5 in primates.

Europe PMC ID: 17012248

Nguyen et al. 2006. Proc Natl Acad Sci U S A. 103(20):7765-70. PMID: 16682635.

Nguyen et al. 2006. Proc Natl Acad Sci U S A. 103(20):7765-70. PMID: 16682635.

FACS

Loss of Siglec expression on T lymphocytes during human evolution.

Europe PMC ID: 16682635

Avril et al. 2005. J Biol Chem. 280(20):19843-51. PMID: 15769739.

Siglec-5 (CD170) can mediate inhibitory signaling in the absence of immunoreceptor tyrosine-based inhibitory motif phosphorylation.

Europe PMC ID: 15769739

Connolly et al. 2002. Br J Haematol. 119(1):221-38. PMID: 12358929.

IHC

Human Siglec-5: tissue distribution, novel isoforms and domain specificities for sialic acid-dependent ligand interactions.

Europe PMC ID: 12358929

Cornish et al. 1998. Blood. 92(6):2123-32. PMID: 9731071.

Characterization of siglec-5, a novel glycoprotein expressed on myeloid cells related to CD33.

Europe PMC ID: 9731071


Add a reference

References: 11 entries

Angata et al. 2006. FASEB J. 20(12):1964-73. PMID: 17012248.

Discovery of Siglec-14, a novel sialic acid receptor undergoing concerted evolution with Siglec-5 in primates.

Nguyen et al. 2006. Proc Natl Acad Sci U S A. 103(20):7765-70. PMID: 16682635.

Nguyen et al. 2006. Proc Natl Acad Sci U S A. 103(20):7765-70. PMID: 16682635.

FACS

Loss of Siglec expression on T lymphocytes during human evolution.

Avril et al. 2005. J Biol Chem. 280(20):19843-51. PMID: 15769739.

Siglec-5 (CD170) can mediate inhibitory signaling in the absence of immunoreceptor tyrosine-based inhibitory motif phosphorylation.

Connolly et al. 2002. Br J Haematol. 119(1):221-38. PMID: 12358929.

IHC

Human Siglec-5: tissue distribution, novel isoforms and domain specificities for sialic acid-dependent ligand interactions.

Cornish et al. 1998. Blood. 92(6):2123-32. PMID: 9731071.

Characterization of siglec-5, a novel glycoprotein expressed on myeloid cells related to CD33.


Add a reference


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