MRP-14 (S100a9 -/-) Knockout Mouse
Invented by Prof Nancy Hogg from Cancer Research UK London Research Institute: Lincoln's Inn Fields
Invented at Cancer Research UK London Research Institute: Lincoln's Inn Fields
- Datasheet
- References (8)
- Inventor Info
Info
| Catalogue Number | 151588 |
| Antigen/Gene or Protein Targets | S100a9 |
| Disease Keywords | Inflammatory disease; |
| Synonyms | Myeloid-related protein 14 (MRP-14) |
| Model | Mutant |
| Relevance | S100a9 and its heterodimeric partner, S100a8, are a cytosolic calcium-binding protein, highly expressed in neutrophils and monocytes. This knockout mice generated by targeted disruption of the gene is useful for understanding the function of S100a9 and the dispensability of both S100a8 and S100a9 for myeloid cell functions. |
| Production Details | The S100a9 gene was disrupted in ES cells with a replacement-type targeting vector and the strategy shown in the publication. ES cells were electroporated, and of 196 neomycin (G418)-resistant clones, 13 were correctly targeted. Two targeted clones were injected into C57BL/6 mouse blastocysts, and two independent strains of S100a9 -/- mice were established. The S100a9 -/- wild-type and mutant alleles were distinguished both by Southern hybridization with probe E following BglII digestion of mouse tail DNA and by a PCR-based assay. Interbreeding of S100a9 heterozygotes yielded the proportions of 1.0:1.9:1.0 for wild-type–heterogeneous–null mice, approximating the expected ratio for Mendelian inheritance. |
| Growth/Phenotype Keywords | No phenotype as yet |
| Strain | C57BL/6 |
| Research Area | Cancer, Cell Structure and Motility, Genetic Studies Tools, Immunology |
References: 8 entries
De Filippo et al. 2014. FASEB J. 28(8):3600-8. PMID: 24776746.
McNeill et al. 2014. Int J Cancer. 135(4):798-808. PMID: 24436096.
A new protective role for S100A9 in regulation of neutrophil recruitment during invasive pneumococcal pneumonia.
Europe PMC ID: 24776746
S100A9 has a protective role in inflammation-induced skin carcinogenesis.
Europe PMC ID: 24436096
Croce et al. 2009. Circulation. 120(5):427-36. PMID: 19620505.
Myeloid-related protein-8/14 is critical for the biological response to vascular injury.
Europe PMC ID: 19620505
Hobbs et al. 2003. Mol Cell Biol. 23(7):2564-76. PMID: 12640137.
Myeloid cell function in MRP-14 (S100A9) null mice.
Europe PMC ID: 12640137
Add a reference
References: 8 entries
De Filippo et al. 2014. FASEB J. 28(8):3600-8. PMID: 24776746.
McNeill et al. 2014. Int J Cancer. 135(4):798-808. PMID: 24436096.
A new protective role for S100A9 in regulation of neutrophil recruitment during invasive pneumococcal pneumonia.
S100A9 has a protective role in inflammation-induced skin carcinogenesis.
Croce et al. 2009. Circulation. 120(5):427-36. PMID: 19620505.
Myeloid-related protein-8/14 is critical for the biological response to vascular injury.
Hobbs et al. 2003. Mol Cell Biol. 23(7):2564-76. PMID: 12640137.
Myeloid cell function in MRP-14 (S100A9) null mice.
Add a reference
