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Polyclonal Antibodies

Anti-Phospho RacGAP1 (Ser157), Polyclonal [pS157 HsCyk-4]

Invented at Cancer Research UK London Research Institute: Clare Hall Laboratories
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Image thumbnail for Anti-Phospho RacGAP1 (Ser157), Polyclonal [pS157 HsCyk-4]
Image thumbnail for Anti-Phospho RacGAP1 (Ser157), Polyclonal [pS157 HsCyk-4]
Image thumbnail for Anti-Phospho RacGAP1 (Ser157), Polyclonal [pS157 HsCyk-4]
Image thumbnail for Anti-Phospho RacGAP1 (Ser157), Polyclonal [pS157 HsCyk-4]
Image thumbnail for Anti-Phospho RacGAP1 (Ser157), Polyclonal [pS157 HsCyk-4]
Image thumbnail for Anti-Phospho RacGAP1 (Ser157), Polyclonal [pS157 HsCyk-4]
Image thumbnail for Anti-Phospho RacGAP1 (Ser157), Polyclonal [pS157 HsCyk-4]
Image thumbnail for Anti-Phospho RacGAP1 (Ser157), Polyclonal [pS157 HsCyk-4]
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Putative molecular mechanism
Plk1 phosphorylates the N-terminal region of HsCyk-4 in vitro
Peptide array identifies a cluster of Plk1 target sites on HsCyk-4
Phosphorylation of HsCyk-4 Ser157 by Plk1 at the central spindle
Ser157 of HsCyk-4 is phosphorylation site in cells. nti-Phospho RacGAP1 (Ser157), Polyclonal [pS157 HsCyk-4] recognize phosphorylated HsCyk-4 in cell extracts.
Immunoprecipitation of HsCyk-4 with anti-Phospho RacGAP1 (Ser157), Polyclonal [pS157 HsCyk-4].
Phosphorylation of HsCyk-4 Ser157 by Plk1 at the central spindle. Western blotting and immunocytochemistry using anti-Phospho RacGAP1 (Ser157), Polyclonal [pS157 HsCyk-4].
Phosphorylation of HsCyk-4 Ser157 by Plk1 at the midbody. Immunofluorescence using anti-Phospho RacGAP1 (Ser157), Polyclonal [pS157 HsCyk-4].
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Image thumbnail for Anti-Phospho RacGAP1 (Ser157), Polyclonal [pS157 HsCyk-4] Image thumbnail for Anti-Phospho RacGAP1 (Ser157), Polyclonal [pS157 HsCyk-4] Image thumbnail for Anti-Phospho RacGAP1 (Ser157), Polyclonal [pS157 HsCyk-4] Image thumbnail for Anti-Phospho RacGAP1 (Ser157), Polyclonal [pS157 HsCyk-4] Image thumbnail for Anti-Phospho RacGAP1 (Ser157), Polyclonal [pS157 HsCyk-4] Image thumbnail for Anti-Phospho RacGAP1 (Ser157), Polyclonal [pS157 HsCyk-4] Image thumbnail for Anti-Phospho RacGAP1 (Ser157), Polyclonal [pS157 HsCyk-4] Image thumbnail for Anti-Phospho RacGAP1 (Ser157), Polyclonal [pS157 HsCyk-4]
Catalogue Number 151587
Applications IF IP WB
Antigen/Gene or Protein Targets pS157 HsCyk-4 (MgcRacGAP and RacGAP1)
Synonyms Cyk-4
Reactivity Human, Mouse and Rat
Relevance HsCyk-4 (or MgcRacGAP) is a subunit of the centralspindlin, and contains a GTPase activator protein (GAP) domain and directly interacts with the GTP exchange factor (GEF) Ect2, and both HsCyk-4 and Ect2 are essential for equatorial recruitment of the RhoA GTPase, the master regulator of actomyosin dynamics during cytokinesis. Plk1 binds and directly phosphorylates the HsCyk-4.
Host Rabbit
Immunogen Synthetic phospho-peptide ILSDI-pS-FDKTD corresponding to HsCyk-4 aa 152-162 (human)
Notes The immunogen is 100% conserved from human to mouse to rat; and the phosphorylation of Ser157 has been shown in human cells but not in mouse and rat.

Western blot 1:1000
Immunoprecipitation
Immunofluorescence 1:500
Positive Control Western blot on mitotic HeLa cell lysates (e.g. nocodazole arrested cells)
Research Area Cell Cycle

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Inventor Information