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|Applications||IF IP WB|
|Antigen/Gene or Protein Targets||pS157 HsCyk-4 (MgcRacGAP and RacGAP1)|
|Reactivity||Human, Mouse and Rat|
|Relevance||HsCyk-4 (or MgcRacGAP) is a subunit of the centralspindlin, and contains a GTPase activator protein (GAP) domain and directly interacts with the GTP exchange factor (GEF) Ect2, and both HsCyk-4 and Ect2 are essential for equatorial recruitment of the RhoA GTPase, the master regulator of actomyosin dynamics during cytokinesis. Plk1 binds and directly phosphorylates the HsCyk-4.|
|Immunogen||Synthetic phospho-peptide ILSDI-pS-FDKTD corresponding to HsCyk-4 aa 152-162 (human)|
The immunogen is 100% conserved from human to mouse to rat; and the phosphorylation of Ser157 has been shown in human cells but not in mouse and rat.
Western blot 1:1000
|Positive Control||Western blot on mitotic HeLa cell lysates (e.g. nocodazole arrested cells)|
|Research Area||Cell Cycle|
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