Log in Register

MEF RAF1 KO Cell Line


Catalogue Number 151576
Antigen/Gene or Protein Targets RAF1
Host Mouse
Tissue Embryo
Disease Keywords Cancer
Model Knock-Out
Relevance The MEF RAF1 KO Cell Line allows in vivo study of Raf-1 knockout and Ras signalling and can be used to carry out drug screening toxicology studies. MEF are produced from the Raf-1+/- knockout mouse.
Production Details Embryos were dissected and cut, washed several times with cold PBS and then incubated at 4°C for 2 hours in 0.25% trypsin. Cells were incubated as per the growth conditions. Primary cells were immortalised and resulting cells grown out by continuous culture.
Research Area Cancer, Cell Signaling & Signal Transduction, Drug Discovery & Development, Metabolism
Recommended Growing Conditions DMEM, 10% FCS, 100U/mol penicillin/streptomycin
Notes See the Raf-1 knockout mouse in related products.
Cellosaurus ID CVCL_6D89

References: 1 entry

Hüser et al. 2001. EMBO J. 20(8):1940-51. PMID: 11296227.

Add a reference

This reagent does not have any reviews at the moment.