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Anti-CD44v3 [2C5]

Info

Catalogue Number 151572
Applications FACS IHC IP WB
Antigen/Gene or Protein Targets CD44v3-10-Fc
Synonyms CD44 Molecule; Hematopoietic Cell E- And L-Selectin Ligand; GP90 Lymphocyte Homing/Adhesion Receptor; Chondroitin Sulfate Proteoglycan 8; Extracellular Matrix Receptor III; Heparan Sulfate Proteoglycan; Phagocytic Glycoprotein; Hyaluronate Receptor; Hermes Antigen; ECMR-III; HUTCH-I; Epican; CDW44; MDU2; MDU3; MIC4; LHR; Cell Surface Glycoprotein CD44; Phagocytic Glycoprotein I; Soluble CD44; CSPG8; HCELL; PGP-1; MC56; Pgp1
Reactivity Human
Relevance Monoclonal antibody which binds all isoforms of CD44.

Background and Research Application
CD44H, also called CD44s, refers to the standard form of CD44 without variable exons. A large number of CD44 isoforms can be generated by the insertion of different combinations of at least nine exons. CD44 is an integral cell membrane glycoprotein that binds to hyaluronan and is involved in matrix adhesion, lymphocyte activation, and lymph node homing. The CD44 protein is expressed as a family of molecular isoforms generated from alternative RNA splicing and posttranslational modifications. CD44 is expressed on multiple cell types and is involved in multiple functions including cell-cell interactions and cell-extracellular matrix binding. CD44 participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, haematopoiesis, and tumour metastasis. Hyaluronan, a high molecular weight polysaccharide component of the extracellular matrix acts as the principal ligand for the CD44 receptor.
Host Mouse
Immunogen Human chimeric fusion protein
Immunogen UniProt ID P16070
Subclass IgG2a
Recommended Growing Conditions DMEM + 10% FBS
Notes Production Details
Purified using multi-step affinity chromatography with protein A.

Storage Conditions
Store at -20 degrees frozen. Avoid repeated freeze/thaw cycles.

Points of Interest
This antibody recognizes all CD44 protein isoforms.

Concentration
1mg/ml as standard
Research Area Adhesion, Cancer, Cell Type or Organelle Marker, Cell Signaling & Signal Transduction, Immunology

References

There are 14 reference entries for this reagent.

View All References

References: 14 entries

Nagano et al. 2004. Cancer Sci. 95(12):930-5. PMID: 15596040.

Lynch et al. 2004. Nat Rev Immunol. 4(12):931-40. PMID: 15573128.

Consequences of regulated pre-mRNA splicing in the immune system.

Europe PMC ID: 15573128

Mechanism and biological significance of CD44 cleavage.

Europe PMC ID: 15596040

Murakami et al. 2003. Oncogene. 22(10):1511-6. PMID: 12629514.

Presenilin-dependent gamma-secretase activity mediates the intramembranous cleavage of CD44.

Europe PMC ID: 12629514

Ponta et al. 2003. Nat Rev Mol Cell Biol. 4(1):33-45. PMID: 12511867.

CD44: from adhesion molecules to signalling regulators.

Europe PMC ID: 12511867

Yu et al. 1996. J Biol Chem. 271(34):20603-7. PMID: 8702806.

A new alternatively spliced exon between v9 and v10 provides a molecular basis for synthesis of soluble CD44.

Europe PMC ID: 8702806

Fox et al. 1994. Cancer Res. 54(16):4539-46. PMID: 7519124.

Normal human tissues, in addition to some tumors, express multiple different CD44 isoforms.

Europe PMC ID: 7519124

Screaton et al. 1992. Proc Natl Acad Sci U S A. 89(24):12160-4. PMID: 1465456.

Genomic structure of DNA encoding the lymphocyte homing receptor CD44 reveals at least 12 alternatively spliced exons.

Europe PMC ID: 1465456


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References: 14 entries

Nagano et al. 2004. Cancer Sci. 95(12):930-5. PMID: 15596040.

Lynch et al. 2004. Nat Rev Immunol. 4(12):931-40. PMID: 15573128.

Consequences of regulated pre-mRNA splicing in the immune system.

Mechanism and biological significance of CD44 cleavage.

Murakami et al. 2003. Oncogene. 22(10):1511-6. PMID: 12629514.

Presenilin-dependent gamma-secretase activity mediates the intramembranous cleavage of CD44.

Ponta et al. 2003. Nat Rev Mol Cell Biol. 4(1):33-45. PMID: 12511867.

CD44: from adhesion molecules to signalling regulators.

Yu et al. 1996. J Biol Chem. 271(34):20603-7. PMID: 8702806.

A new alternatively spliced exon between v9 and v10 provides a molecular basis for synthesis of soluble CD44.

Fox et al. 1994. Cancer Res. 54(16):4539-46. PMID: 7519124.

Normal human tissues, in addition to some tumors, express multiple different CD44 isoforms.

Screaton et al. 1992. Proc Natl Acad Sci U S A. 89(24):12160-4. PMID: 1465456.

Genomic structure of DNA encoding the lymphocyte homing receptor CD44 reveals at least 12 alternatively spliced exons.


Add a reference

Inventor Information

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