- Reviews (0)
|Antigen/Gene or Protein Targets||POT1|
|Reactivity||Human and Mouse|
|Relevance||POT1 (protection of telomeres 1) is part of the telomerase ribonucleotide (RNP) complex, which stabilises chromosomes by controlling telomere elongation. POT1 is a component of the double-stranded telomeric DNA-binding TRF1 complex which cis-inhibits telomerase. POT1 localization on telomeres diminishes in the absence of single-stranded DNA.|
|Immunogen||Full-length human POT1 protein|
|Formulation||PBS, 0.1% NaN3|
|Molecular Weight (kDa)||70|
Available as immunogen affinity purified.
Recommended immunostaining protocol:
Samples (50-100 μg) are separated by SDS-PAGE on 10% Bis-Tris gels for 1 to 1.5 hours at 180V and then transferred onto nitrocellulose membrane at 30V for 100 minutes. Blocking is performed for a minimum of 30 minutes in 5% milk in PBS-Tween and the MTA40 antibody is left for overnight incubation. The membrane is then washed in PBS-Tween and the secondary antibody (Third party manufacturer) is added for a minimum of one hour. After washing in PBS-Tween, the immunostaining is visualised with ECL reaction.
|Positive Control||U2OS cells|
|Research Area||Cancer, Epigenetics & Nuclear Signalling|
Have you used this reagent? Share your experience by submitting a review and help your fellow scientists.Review this reagent