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Anti-POT1, Polyclonal [MTA40]

Info

Catalogue Number 151570
Applications IP WB
Antigen/Gene or Protein Targets POT1
Reactivity Human and Mouse
Relevance POT1 (protection of telomeres 1) is part of the telomerase ribonucleotide (RNP) complex, which stabilises chromosomes by controlling telomere elongation. POT1 is a component of the double-stranded telomeric DNA-binding TRF1 complex which cis-inhibits telomerase. POT1 localization on telomeres diminishes in the absence of single-stranded DNA.
Host Rabbit
Immunogen Full-length human POT1 protein
Concentration 1.5 mg/ml
Formulation PBS, 0.1% NaN3
Molecular Weight (kDa) 70
Notes Available as immunogen affinity purified.
Recommended immunostaining protocol:
Samples (50-100 μg) are separated by SDS-PAGE on 10% Bis-Tris gels for 1 to 1.5 hours at 180V and then transferred onto nitrocellulose membrane at 30V for 100 minutes. Blocking is performed for a minimum of 30 minutes in 5% milk in PBS-Tween and the MTA40 antibody is left for overnight incubation. The membrane is then washed in PBS-Tween and the secondary antibody (Third party manufacturer) is added for a minimum of one hour. After washing in PBS-Tween, the immunostaining is visualised with ECL reaction.
Positive Control U2OS cells
Research Area Cancer, Epigenetics & Nuclear Signalling

References: 2 entries

Martinez et al. 2010. Nat Cell Biol. 12(8):768-80. PMID: 20622869.

Martínez et al. 2009. Genes Dev. 23(17):2060-75. PMID: 19679647.


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