LSLBRafD594A Mouse
Invented at University Of Leicester
- Datasheet
- References (2)
- Inventor Info
Info
| Catalogue Number | 151566 |
| Antigen/Gene or Protein Targets | Mouse BRAF |
| Disease Keywords | Cancer, Malignant Melanoma |
| Relevance | Mimics the expression of D594ABRAF in human cancer samples. In conjunction with oncogenic G12DKRAS, this mutant induces the development of highly aggressive malignant melanomas. Conditional knockin (Cre-lox) D594A mutation of BRaf |
| Production Details | A transgenic vector was generated and used to modify the endogenous gene in E14.1a embryonic stem cells by homologous recombination. Targeted ES cells were microinjected into C57BL6 blastocysts and chimaeras were derived. These were bred to C57BL6 mice to obtain germline transmission, from which a colony of heterozygous LSL-BrafD594A mice on the C57BL6 background were derived. |
| Conditional | Yes |
| Conditional Description | Cre-lox |
| Growth/Phenotype Keywords | Progressive aneuploidy |
| Research Area | Cancer, Genetic Studies Tools |
References: 2 entries
Heidorn et al. 2010. Cell. 140(2):209-21. PMID: 20141835.
Kinase-dead BRAF and oncogenic RAS cooperate to drive tumor progression through CRAF.
Europe PMC ID: 20141835
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References: 2 entries
Heidorn et al. 2010. Cell. 140(2):209-21. PMID: 20141835.
Kinase-dead BRAF and oncogenic RAS cooperate to drive tumor progression through CRAF.
Add a reference
