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LSLBRafD594A Mouse


Catalogue Number 151566
Antigen/Gene or Protein Targets Mouse BRAF
Disease Keywords Cancer, Malignant Melanoma
Relevance Mimics the expression of D594ABRAF in human cancer samples. In conjunction with oncogenic G12DKRAS, this mutant induces the development of highly aggressive malignant melanomas. Conditional knockin (Cre-lox) D594A mutation of BRaf
Production Details A transgenic vector was generated and used to modify the endogenous gene in E14.1a embryonic stem cells by homologous recombination. Targeted ES cells were microinjected into C57BL6 blastocysts and chimaeras were derived. These were bred to C57BL6 mice to obtain germline transmission, from which a colony of heterozygous LSL-BrafD594A mice on the C57BL6 background were derived.
Conditional Yes
Conditional Description Cre-lox
Growth/Phenotype Keywords Progressive aneuploidy
Research Area Cancer, Genetic Studies Tools

References: 1 entry

Heidorn et al. 2010. Cell. 140(2):209-21. PMID: 20141835.

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