Anti-CDX1 [123a]
Invented by Prof Walter Bodmer from University of Oxford
Invented at University of Oxford
- Datasheet
- References (10)
- Inventor Info
Info
| Catalogue Number | 151546 |
| Applications | ChIP IF WB ChIP-seq |
| Antigen/Gene or Protein Targets | CDX1 |
| Synonyms | Caudal Type Homeobox 1 |
| Reactivity | Human |
| Relevance | CDX1 is an intestine-specific transcription factor with a role in directing intestinal development, differentiation, proliferation and maintenance of the intestinal phenotype. Cdx1 positively regulates its own expression in the small intestine and colon of fetus and adult and promotes cellular growth and differentiation in epithelial intestinal cells. A reduction in human Cdx1 expression is associated with colorectal tumorigenesis. Both Cdx1 and Cdx2 genes must be expressed to reduce tumorigenic potential, to increase sensitivity to apoptosis and to reduce cell migration, suggesting that the two genes control the normal phenotype by independent pathways. |
| Host | Mouse |
| Immunogen | Human CDX-1 N-terminal peptide |
| Positive Control | HT55, LS174T, RCM1 |
| Subclass | IgG1 |
| Molecular Weight (kDa) | 32 |
| Myeloma Used | P3/NS1/1-Ag4.1 |
| Recommended Growing Conditions | RPMI 1640 + 10% FCS + HAT |
| Strain | Balb/c |
| Notes | Notes from originator: Clone 123a has a degree of cross reactivity with a determinant of different molecular weight when applied by WB and IHC in fibroblasts and smooth muscle cells. The cross reacting product does not appear in epithelial cells (or at least is significantly weaker). |
| Research Area | Apoptosis and Programmed Cell Death, Cancer, Epigenetics & Nuclear Signalling |
References: 10 entries
Jones et al. 2015. Proc Natl Acad Sci U S A. 112(13):E1550-8. PMID: 25775580.
ChIP ChIP-seq
The CDX1-microRNA-215 axis regulates colorectal cancer stem cell differentiation.
Europe PMC ID: 25775580
Ashley et al. 2013. Cancer Res. 73(18):5798-809. PMID: 23867471.
Stem cell differentiation and lumen formation in colorectal cancer cell lines and primary tumors.
Europe PMC ID: 23867471
Yeung et al. 2011. Proc Natl Acad Sci U S A. 108(11):4382-7. PMID: 21368208.
IF
Hypoxia and lineage specification of cell line-derived colorectal cancer stem cells.
Europe PMC ID: 21368208
Yeung et al. 2010. Proc Natl Acad Sci U S A. 107(8):3722-7. PMID: 20133591.
IF
Cancer stem cells from colorectal cancer-derived cell lines.
Europe PMC ID: 20133591
Chan et al. 2009. Proc Natl Acad Sci U S A. 106(6):1936-41. PMID: 19188603.
ChIP WB
Gastrointestinal differentiation marker Cytokeratin 20 is regulated by homeobox gene CDX1.
Europe PMC ID: 19188603
Add a reference
References: 10 entries
Jones et al. 2015. Proc Natl Acad Sci U S A. 112(13):E1550-8. PMID: 25775580.
ChIP ChIP-seq
The CDX1-microRNA-215 axis regulates colorectal cancer stem cell differentiation.
Ashley et al. 2013. Cancer Res. 73(18):5798-809. PMID: 23867471.
Stem cell differentiation and lumen formation in colorectal cancer cell lines and primary tumors.
Yeung et al. 2011. Proc Natl Acad Sci U S A. 108(11):4382-7. PMID: 21368208.
IF
Hypoxia and lineage specification of cell line-derived colorectal cancer stem cells.
Yeung et al. 2010. Proc Natl Acad Sci U S A. 107(8):3722-7. PMID: 20133591.
IF
Cancer stem cells from colorectal cancer-derived cell lines.
Chan et al. 2009. Proc Natl Acad Sci U S A. 106(6):1936-41. PMID: 19188603.
ChIP WB
Gastrointestinal differentiation marker Cytokeratin 20 is regulated by homeobox gene CDX1.
Add a reference
