Anti-Rheb [Rheb 3H6]
Invented at The Institute of Cancer Research
- Datasheet
- References (2)
- Inventor Info
Info
| Catalogue Number | 151544 |
| Applications | IP WB |
| Antigen/Gene or Protein Targets | Rheb |
| Reactivity | Human |
| Relevance |
Monoclonal antibody which binds the Rheb GTP binding protein, and can be used to help understand the role of Rheb in mTORC signalling. Background and Research Application Rheb (Ras homolog enriched in brain) is an evolutionarily conserved member of the Ras superfamily of small GTP binding proteins. Rheb is expressed at high levels in the brain, but is also found in many other tissues, and can be induced by growth factor stimulation. It is ubiquitously expressed in humans and other mammals. Rheb triggers activation of the Raf-MEK-MAPK pathway and has an important role in regulating the insulin/Target of rapamycin (TOR) signalling pathway, and the cell cycle. Rheb stimulates the phosphorylation of S6K1 and EIF4EBP1 through activation of mTORC1 signalling. Overexpression of Rheb can be seen in multiple human carcinomas. |
| Host | Mouse |
| Immunogen | Human Rheb GST fusion |
| Immunogen UniProt ID | Q15382 |
| Subclass | IgG1 |
| Recommended Growing Conditions | DMEM + 5%FCS |
| Notes |
Production Details Purified using multi-step affinity chromatography with protein A. Storage Conditions Store at -20 degrees frozen. Avoid repeated freeze/thaw cycles. Points of Interest Suggested Dilutions: Western blot: 1ug/ml Immunoprecipitation: 5ug/ml Concentration 1mg/ml as standard |
| Research Area | Cancer, Cell Signaling & Signal Transduction, Metabolism |
References: 2 entries
Findlay et al. 2007. Biochem J. 403(1):13-20. PMID: 17253963.
A MAP4 kinase related to Ste20 is a nutrient-sensitive regulator of mTOR signalling.
Europe PMC ID: 17253963
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References: 2 entries
Findlay et al. 2007. Biochem J. 403(1):13-20. PMID: 17253963.
A MAP4 kinase related to Ste20 is a nutrient-sensitive regulator of mTOR signalling.
Add a reference
