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Anti-β1 Connexin [2A]

Info

Catalogue Number 151524
Applications IHC IF WB
Antigen/Gene or Protein Targets Connexin 32 (Cx32 or ß1)
Reactivity Chicken, Human, Mouse and Rat
Relevance Monoclonal antibody which detects connexin 32, linked to Charcot-Marie-Tooth disease.

Background and Research Application
Connexins are a large family of proteins which form gap junctions. Gap junctions are cell membrane structures that facilitate direct cell-cell communication. Gap junctions are essential for various physiological processes, including coordinated depolarization of cardiac muscle, proper embryonic development, and the conducted response in microvasculature. Gap junction communication has been implicated in the patterning and development of vertebrate embryos. Individual connexins show complex spatial and temporal variation in expression patterns. This variation contributes to the control of intercellular signalling.

Mutations in connexin 32 are linked to Charcot-Marie-Tooth disease X-linked type 1 (CMTX1), the most commonly inherited disorder of the peripheral nervous system.
Host Mouse
Immunogen The peptide, LEGHGDPLHLEE, was coupled at the C-terminal end to bovine thyroglobulin at a final molar ratio peptide:thyroglobulin of 40–60:1.
Immunogen UniProt ID P08034
Positive Control Heart & liver tissue from 6 week old mice
Subclass IgG
Molecular Weight (kDa) 46
Myeloma Used P3X63Ag8.653
Recommended Growing Conditions RPMI 1640 + 10-20% FBS + glutamine (2mM)
Notes Production Details
Purified using multi-step affinity chromatography with protein A.

Storage Conditions
Store at -20 degrees frozen. Avoid repeated freeze/thaw cycles.

Points of Interest
Connexin 32 gene has been mapped to Xq13.1. It consists of two exons and encodes a gap junction protein containing four transmembrane domains.

Concentration
1mg/ml as standard
Research Area Cell Signaling & Signal Transduction, Neurobiology

References

There are 2 reference entries for this reagent.

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References: 2 entries

Wright et al. 2001. Reproduction. 121(1):77-88. PMID: 11226030.

Stage-specific and differential expression of gap junctions in the mouse ovary: connexin-specific roles in follicular regulation.

Europe PMC ID: 11226030


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References: 2 entries

Wright et al. 2001. Reproduction. 121(1):77-88. PMID: 11226030.

Stage-specific and differential expression of gap junctions in the mouse ovary: connexin-specific roles in follicular regulation.


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Inventor Information

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