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Monoclonal Antibodies

Anti-α1 Connexin [1A]

Invented at University College London
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Adapted from Pearson RA, Lüneborg NL, Becker DL, Mobbs P. Gap junctions modulate interkinetic nuclear movement in retinal progenitor cells. J Neurosci. 2005 Nov 16;25(46):10803-14. doi: 10.1523/JNEUROSCI.2312-05.2005. PMID: 16291954; PMCID: PMC6725838.The effects of gap-junction blockers on interkinetic nuclear movement. G, Cx43 staining (red) in controls (left) and after application of Cx43 asODNs (right). The preparations were counterstained with Hoechst 33342 (blue) to label the nuclei. Images are projections of 10 confocal sections taken at 1 μm steps through the ventricular zone of each retinal preparation. For additional details of the extent of connexin knockdown, see Materials and Methods. Scale bar, 10 μm.
Adapted from Wright, CS, Becker, DL, Lin, JS, Warner, AE, & Hardy, K. (2001). Stage-specific and differential expression of gap junctions in the mouse ovary: connexin-specific roles in follicular regulation, Reproduction, 121(1), 77-88. PMID: 11226030. Connexin antibodies 1A, 2A and anti-β4 recognize their parent proteins. (A) Monoclonal antibody 1A identifies the intercalated discs (arrowheads) and punctate gap junctions composed of α1 connexin (Cx43) that lie in the parallel membranes of myocytes in a frozen section of rat heart. The panel to the right shows the single clear band at 43 kDa identified on a western blot against protein isolated from rat heart. The band is relatively broad and may include the minor bands that indicate the phosphorylated forms of the connexin protein.
Adapted from Wright, CS, Becker, DL, Lin, JS, Warner, AE, & Hardy, K. (2001). Stage-specific and differential expression of gap junctions in the mouse ovary: connexin-specific roles in follicular regulation, Reproduction, 121(1), 77-88. PMID: 11226030. Distribution of connexin types within gap junctions of adult and prepubertal mouse ovarian follicles. Nuclei are stained with propidium iodide (red) and connexins are stained with fluorescein isothiocyanate (FITC) (green). (A) Immunolabelling of α1 connexin. (Cx43)-containing gap junctions with Gap 15 between granulosa cells of secondary follicles in the central region of a prepubertal ovary. (B) Immunolabelling (arrowhead) of α1 connexin with Gap 15 showing expression restricted to the granulosa (g) of inner secondary follicles (28) but not outer primary follicles (18) in a prepubertal ovary. (C) Immunolabelling of α1 connexin in an adult ovary with 1A in gap junctions between granulosa cells (g) at several different follicular stages, showing strong expression in large follicles (arrow) and weaker expression in smaller follicles (arrowhead) t: theca. (D) Immunolabelling of α1 connexin with Gap 15 in a large antral follicle from an adult ovary showing gap junctions between granulosa cells (g) but not thecal cells (t). (E) Immunolabelling of α4 connexin (Cx37) with anti-α4(DP) on the oocyte (o) surface (arrowhead) but not between granulosa cells (g) in an adult preantral follicle, and (inset) between granulosa cells in a larger follicle. (F) Immunolabelling of α4 connexin with Gap 23 between granulosa cells (g) in a large antral follicle of an adult ovary. Scale bars represent 50 µm.
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Catalogue Number 151523
Applications IHC IF WB
Antigen/Gene or Protein Targets Connexin 43 (Cx43 or α1)
Reactivity Mouse
Relevance Connexins are a large family of proteins which form gap junctions. Gap junctions are cell membrane structures that facilitate direct cell-cell communication. Gap junction communication has been implicated in the patterning and development of vertebrate embryos. Individual connexins show complex spatial and temporal variation in expression patterns. This variation contributes to the control of intercellular signalling.
Host Mouse
Immunogen The peptide, EIKKFKYGIEEH, was coupled at the C-terminal end to bovine thyroglobulin at a final molar ratio peptide:thyroglobulin of 40–60:1.
Positive Control Heart & liver tissue from 6 week old mice; Heart tissue expresses predominantly Cx43
Subclass IgG
Myeloma Used P3X63Ag8.653
Recommended Growing Conditions RPMI 1640 + 10-20% FBS + glutamine (2mM)
Research Area Cell Signaling & Signal Transduction, Neurobiology

References

There are 8 reference entries for this reagent.

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References: 8 entries

Gorbe et al. 2007. Exp Cell Res. 313(6):1135-48. PMID: 17331498.

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Myoblast proliferation and syncytial fusion both depend on connexin43 function in transfected skeletal muscle primary cultures.

Europe PMC ID: 17331498

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Pearson et al. 2005. J Neurosci. 25(46):10803-14. PMID: 16291954.

View  

Gap junctions modulate interkinetic nuclear movement in retinal progenitor cells.

Europe PMC ID: 16291954

View  

Saitongdee et al. 2004. J Histochem Cytochem. 52(5):603-15. PMID: 15100238.

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Levels of gap junction proteins in coronary arterioles and aorta of hamsters exposed to the cold and during hibernation and arousal.

Europe PMC ID: 15100238

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Wright et al. 2001. Reproduction. 121(1):77-88. PMID: 11226030.

View  

Stage-specific and differential expression of gap junctions in the mouse ovary: connexin-specific roles in follicular regulation.

Europe PMC ID: 11226030

View  

Add a reference

References: 8 entries

Gorbe et al. 2007. Exp Cell Res. 313(6):1135-48. PMID: 17331498.

View  

Myoblast proliferation and syncytial fusion both depend on connexin43 function in transfected skeletal muscle primary cultures.

View  

Pearson et al. 2005. J Neurosci. 25(46):10803-14. PMID: 16291954.

View  

Gap junctions modulate interkinetic nuclear movement in retinal progenitor cells.

View  

Saitongdee et al. 2004. J Histochem Cytochem. 52(5):603-15. PMID: 15100238.

View  

Levels of gap junction proteins in coronary arterioles and aorta of hamsters exposed to the cold and during hibernation and arousal.

View  

Wright et al. 2001. Reproduction. 121(1):77-88. PMID: 11226030.

View  

Stage-specific and differential expression of gap junctions in the mouse ovary: connexin-specific roles in follicular regulation.

View  

Add a reference

Inventor Information

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Invented at

University College London
University College London

49 reagents listed on Ximbio