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CDH5(PAC)CreERT2 Mouse


Catalogue Number 151520
Antigen/Gene or Protein Targets Estrogen receptor (ERT2) under the vascular endothelial cadherin (Cdh5(PAC)) promoter.
Disease Keywords Cancer; angiogenesis;
Relevance Cdh5(PAC)-CreERT2 enable efficient inducible conditional recombinase expression in embryonic and adult endothelial cells (tissue-specific loxP knockout/knockin/transgene). The Cdh5(PAC)-CreERT2 mouse is an ideal tool in the study of gene function in angiogenesis, atherosclerosis and neovascularisation.
Production Details A Cdh5(PAC)-CreERT2 transgene vector, containing a genomic Cdh5(PAC) promoter fragment fused to a CreERT2 cDNA, was injected into fertilised embryos (C57BL/6 or FVB/N). Founder lines were back-crossed to establish mice heterozygous for the Cdh5(PAC)-CreERT2 transgene.
Conditional Description Conditional Cre-ERT2 expression under Cdh5(PAC) promoter in endothelial cells; inducible Cre activity by treatment with hormone (tamoxifen), inducing translocation of Cre-ERT2 to nucleus.
Mouse Genetic Background/Cross History C57BL6 or FVB/N
Zygosity Heterozygous
Research Area Cancer, Cardiovascular, Developmental Biology, Genetic Studies Tools, Neurobiology
Notes The Cdh5(PAC)-CreERT2 mouse exhibits tissue-specific expression of an inducible Cre-ERT2 fusion protein, enabling tamoxifen-induced Cre recombinase activity in vascular endothelial cells. Administration of tamoxifen induces nuclear translocation of the Cre-ERT2 fusion protein, and subsequent Cre recombinase activity, allowing knockout/knockin/transgene studies of loxP-flanked genes in vascular endothelial cells.

Non-induced Cdh5(PAC)-CreERT2 mice demonstrate no Cre recombinase activity, while tamoxifen-induced Cdh5(PAC)-CreERT2 mice demonstrate high penetrance in vascular endothelial cells (95%+).

References: 9 entries

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Luna-Zurita et al. 2010. J Clin Invest. 120(10):3493-507. PMID: 20890042.

Pitulescu et al. 2010. Nat Protoc. 5(9):1518-34. PMID: 20725067.

Robson et al. 2010. Dev Dyn. 239(9):2435-42. PMID: 20652948.

Wang et al. 2010. Nature. 465(7297):483-6. PMID: 20445537.

Mahmoud et al. 2010. Circ Res. 106(8):1425-33. PMID: 20224041.

Bazigou et al. 2009. Dev Cell. 17(2):175-86. PMID: 19686679.

Benedito et al. 2009. Cell. 137(6):1124-35. PMID: 19524514.

Sörensen et al. 2009. Blood. 113(22):5680-8. PMID: 19144989.

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