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CDH5(PAC)CreERT2 Mouse

Invented by Prof Ralf H. Adams at Max Planck Institute

Info

Catalogue Number 151520
Antigen/Gene or Protein Targets Estrogen receptor (ERT2) under the vascular endothelial cadherin (Cdh5(PAC)) promoter.
Disease Keywords Cancer; angiogenesis;
Relevance Cdh5(PAC)-CreERT2 enable efficient inducible conditional recombinase expression in embryonic and adult endothelial cells (tissue-specific loxP knockout/knockin/transgene). The Cdh5(PAC)-CreERT2 mouse is an ideal tool in the study of gene function in angiogenesis, atherosclerosis and neovascularisation.
Production Details A Cdh5(PAC)-CreERT2 transgene vector, containing a genomic Cdh5(PAC) promoter fragment fused to a CreERT2 cDNA, was injected into fertilised embryos (C57BL/6 or FVB/N). Founder lines were back-crossed to establish mice heterozygous for the Cdh5(PAC)-CreERT2 transgene.
Conditional Description Conditional Cre-ERT2 expression under Cdh5(PAC) promoter in endothelial cells; inducible Cre activity by treatment with hormone (tamoxifen), inducing translocation of Cre-ERT2 to nucleus.
Mouse Genetic Background/Cross History C57BL6 or FVB/N
Zygosity Heterozygous
Research Area Cancer, Cardiovascular, Developmental Biology, Genetic Studies Tools, Neurobiology
Notes The Cdh5(PAC)-CreERT2 mouse exhibits tissue-specific expression of an inducible Cre-ERT2 fusion protein, enabling tamoxifen-induced Cre recombinase activity in vascular endothelial cells. Administration of tamoxifen induces nuclear translocation of the Cre-ERT2 fusion protein, and subsequent Cre recombinase activity, allowing knockout/knockin/transgene studies of loxP-flanked genes in vascular endothelial cells.


Non-induced Cdh5(PAC)-CreERT2 mice demonstrate no Cre recombinase activity, while tamoxifen-induced Cdh5(PAC)-CreERT2 mice demonstrate high penetrance in vascular endothelial cells (95%+).

References: 18 entries

Bréart et al. 2011. J Exp Med. 208(6):1267-78. PMID: 21576386.

Lipid phosphate phosphatase 3 enables efficient thymic egress.

Europe PMC ID: 21576386

Luna-Zurita et al. 2010. J Clin Invest. 120(10):3493-507. PMID: 20890042.

Pitulescu et al. 2010. Nat Protoc. 5(9):1518-34. PMID: 20725067.

Robson et al. 2010. Dev Dyn. 239(9):2435-42. PMID: 20652948.

Integration of a Notch-dependent mesenchymal gene program and Bmp2-driven cell invasiveness regulates murine cardiac valve formation.

Europe PMC ID: 20890042

The TGFβ type II receptor plays a critical role in the endothelial cells during cardiac development.

Europe PMC ID: 20652948

Inducible gene targeting in the neonatal vasculature and analysis of retinal angiogenesis in mice.

Europe PMC ID: 20725067

Wang et al. 2010. Nature. 465(7297):483-6. PMID: 20445537.

Ephrin-B2 controls VEGF-induced angiogenesis and lymphangiogenesis.

Europe PMC ID: 20445537

Mahmoud et al. 2010. Circ Res. 106(8):1425-33. PMID: 20224041.

Pathogenesis of arteriovenous malformations in the absence of endoglin.

Europe PMC ID: 20224041

Bazigou et al. 2009. Dev Cell. 17(2):175-86. PMID: 19686679.

Integrin-alpha9 is required for fibronectin matrix assembly during lymphatic valve morphogenesis.

Europe PMC ID: 19686679

Benedito et al. 2009. Cell. 137(6):1124-35. PMID: 19524514.

The notch ligands Dll4 and Jagged1 have opposing effects on angiogenesis.

Europe PMC ID: 19524514

Sörensen et al. 2009. Blood. 113(22):5680-8. PMID: 19144989.

DLL1-mediated Notch activation regulates endothelial identity in mouse fetal arteries.

Europe PMC ID: 19144989


Add a reference

References: 18 entries

Bréart et al. 2011. J Exp Med. 208(6):1267-78. PMID: 21576386.

Lipid phosphate phosphatase 3 enables efficient thymic egress.

Europe PMC ID: 21576386

Luna-Zurita et al. 2010. J Clin Invest. 120(10):3493-507. PMID: 20890042.

Pitulescu et al. 2010. Nat Protoc. 5(9):1518-34. PMID: 20725067.

Robson et al. 2010. Dev Dyn. 239(9):2435-42. PMID: 20652948.

Integration of a Notch-dependent mesenchymal gene program and Bmp2-driven cell invasiveness regulates murine cardiac valve formation.

Europe PMC ID: 20890042

The TGFβ type II receptor plays a critical role in the endothelial cells during cardiac development.

Europe PMC ID: 20652948

Inducible gene targeting in the neonatal vasculature and analysis of retinal angiogenesis in mice.

Europe PMC ID: 20725067

Wang et al. 2010. Nature. 465(7297):483-6. PMID: 20445537.

Ephrin-B2 controls VEGF-induced angiogenesis and lymphangiogenesis.

Europe PMC ID: 20445537

Mahmoud et al. 2010. Circ Res. 106(8):1425-33. PMID: 20224041.

Pathogenesis of arteriovenous malformations in the absence of endoglin.

Europe PMC ID: 20224041

Bazigou et al. 2009. Dev Cell. 17(2):175-86. PMID: 19686679.

Integrin-alpha9 is required for fibronectin matrix assembly during lymphatic valve morphogenesis.

Europe PMC ID: 19686679

Benedito et al. 2009. Cell. 137(6):1124-35. PMID: 19524514.

The notch ligands Dll4 and Jagged1 have opposing effects on angiogenesis.

Europe PMC ID: 19524514

Sörensen et al. 2009. Blood. 113(22):5680-8. PMID: 19144989.

DLL1-mediated Notch activation regulates endothelial identity in mouse fetal arteries.

Europe PMC ID: 19144989


Add a reference

References: 18 entries

Bréart et al. 2011. J Exp Med. 208(6):1267-78. PMID: 21576386.

Lipid phosphate phosphatase 3 enables efficient thymic egress.

Luna-Zurita et al. 2010. J Clin Invest. 120(10):3493-507. PMID: 20890042.

Pitulescu et al. 2010. Nat Protoc. 5(9):1518-34. PMID: 20725067.

Robson et al. 2010. Dev Dyn. 239(9):2435-42. PMID: 20652948.

Integration of a Notch-dependent mesenchymal gene program and Bmp2-driven cell invasiveness regulates murine cardiac valve formation.

The TGFβ type II receptor plays a critical role in the endothelial cells during cardiac development.

Inducible gene targeting in the neonatal vasculature and analysis of retinal angiogenesis in mice.

Wang et al. 2010. Nature. 465(7297):483-6. PMID: 20445537.

Ephrin-B2 controls VEGF-induced angiogenesis and lymphangiogenesis.

Mahmoud et al. 2010. Circ Res. 106(8):1425-33. PMID: 20224041.

Pathogenesis of arteriovenous malformations in the absence of endoglin.

Bazigou et al. 2009. Dev Cell. 17(2):175-86. PMID: 19686679.

Integrin-alpha9 is required for fibronectin matrix assembly during lymphatic valve morphogenesis.

Benedito et al. 2009. Cell. 137(6):1124-35. PMID: 19524514.

The notch ligands Dll4 and Jagged1 have opposing effects on angiogenesis.

Sörensen et al. 2009. Blood. 113(22):5680-8. PMID: 19144989.

DLL1-mediated Notch activation regulates endothelial identity in mouse fetal arteries.


Add a reference


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