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Catalogue Number
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151519 |
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Antigen/Gene or Protein Targets
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BRaf V600E (oncogenic mutant)
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Disease Keywords
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Cancer; melanoma; colorectal cancer;
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Synonyms
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LSL B-RafV600E
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Model
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Knock-In
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Relevance
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Conditional knockin of oncogenic B-Raf V600E; in vivo study of oncogenic B-raf mutation and signalling pathways The LSL-B-Raf-V600E targeting vector, containing a loxP flanked minigene+polyA encoding wildtype exons 15 to 18, amd a loxP flanked resistance cassette, and a substitution of residue 600 (V to E) in the endogenous exon 15, was transfected into E14.1 ES cells. Properly targeted ES cells containing homologous recombination events were injected into C57BL6 balstocysts. Chimeric mice were backcrossed on the C57BL6 background.
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Conditional
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Yes
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Conditional Description
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B-Raf gene with loxP sites flanking wildtype exons 15 to 18 (plus STOP) inserted before mutant exons 15 (V600E) to 18, enabling conditional Cre-mediate excision of wildtype exons and simultaneous knockin of mutant exons.
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Mouse Genetic Background/Cross History
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E14.1/C57BL6
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Zygosity
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Heterozygous
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Research Area
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Cancer, Cell Signaling & Signal Transduction, Genetic Studies Tools
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