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Monoclonal Antibodies

Anti-Zebrafish gut secretory cell epitopes [FIS 6G5/1]

Invented by Linda Ariza-McNaughton from Francis Crick Institute
Invented at Cancer Research UK London Research Institute: Lincoln's Inn Fields
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Catalogue Number 151514
Applications ELISA IHC IF WB
Antigen/Gene or Protein Targets Zebrafish gut secretory cell epitopes
Reactivity Zebrafish
Relevance The transparency of the juvenile zebrafish and its genetic advantages make it an attractive model for the study of intestinal differentiation and renewal. This antibody labels the secretory cells of the zebrafish intestinal epithelium, both mucous and enteroendocrine, corresponding to the class of cells that are lost in MathI mouse mutants (Yang et al, (2001) Science 294: 2155-2158).
Host Mouse
Immunogen Lysate of zebrafish intestine
Subclass IgG1
Myeloma Used Sp2/0-Ag14
Strain Balb/c
Notes Suggested Dilution for western blot: 1:200.

6G5/1 may be used to stain formalin fixed tissue samples.
Research Area Aquaculture, Cell Cycle, Cell Type or Organelle Marker, Cell Signaling & Signal Transduction, Developmental Biology, Epigenetics & Nuclear Signalling, Epitope Tags, Stem Cell Biology

References

There are 8 reference entries for this reagent.

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References: 8 entries

Matthews et al. 2008. Dev Dyn. 237(1):124-31. PMID: 18095340.

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Transcription factor onecut3 regulates intrahepatic biliary development in zebrafish.

Europe PMC ID: 18095340

View  

Dong et al. 2007. Nat Genet. 39(3):397-402. PMID: 17259985.

View  

Fgf10 regulates hepatopancreatic ductal system patterning and differentiation.

Europe PMC ID: 17259985

View  

Bates et al. 2006. Dev Biol. 297(2):374-86. PMID: 16781702.

View  

Distinct signals from the microbiota promote different aspects of zebrafish gut differentiation.

Europe PMC ID: 16781702

View  

Crosnier et al. 2005. Development. 132(5):1093-104. PMID: 15689380.

View  

Delta-Notch signalling controls commitment to a secretory fate in the zebrafish intestine.

Europe PMC ID: 15689380

View  

Add a reference

References: 8 entries

Matthews et al. 2008. Dev Dyn. 237(1):124-31. PMID: 18095340.

View  

Transcription factor onecut3 regulates intrahepatic biliary development in zebrafish.

View  

Dong et al. 2007. Nat Genet. 39(3):397-402. PMID: 17259985.

View  

Fgf10 regulates hepatopancreatic ductal system patterning and differentiation.

View  

Bates et al. 2006. Dev Biol. 297(2):374-86. PMID: 16781702.

View  

Distinct signals from the microbiota promote different aspects of zebrafish gut differentiation.

View  

Crosnier et al. 2005. Development. 132(5):1093-104. PMID: 15689380.

View  

Delta-Notch signalling controls commitment to a secretory fate in the zebrafish intestine.

View  

Add a reference

Inventor Information