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Catalogue Number 151503
Antigen/Gene or Protein Targets ERBB2
Parental Line MSTV1-7 HER2
Host Human
Tissue Breast
Disease Keywords Breast cancer
Model Transgenic
Relevance The human mammary luminal epithelial cell line MTSV1-7 was transfected with an ERBB2 expression vector, enabling over-expression of the ERBB2 proto-oncogene. It allows in vitro study of ERBB2 proto-oncogene over-expression in human mammary epithelia adn of the effects of ERBB2 over-expression on epithelial morphology & adhesion. The over-expression of the ERBB2 receptor inhibits morphogenesis in vitro, and induces reduced expression of surface adhesion molecules as E-Cadherin and a2 integrin, suggesting a role for ERBB2 over-expression in tumour progression and metastasis.
Production Details MTSV1-7 transfected with SV40 plasmid DNA; MTSV1-7 ce1 line transfected with plasmid constructs for pSV2-erbB2 and pSV2neo.
Research Area Adhesion, Cancer, Cell Signaling & Signal Transduction, Cell Structure and Motility, Drug Discovery & Development
Recommended Growing Conditions DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) + Insulin (bovine) at 10ug/ml +Hycrocortisone at 5ug/ml.
Cellosaurus ID CVCL_3033

References: 6 entries

D'souza et al. 1994. Proc Natl Acad Sci U S A. 91(15):7202-6. PMID: 7913748.

Overexpression of ERBB2 in human mammary epithelial cells signals inhibition of transcription of the E-cadherin gene.

Europe PMC ID: 7913748

D'Souza et al. 1993. Oncogene. 8(7):1797-806. PMID: 8099725.

Collagen-induced morphogenesis and expression of the alpha 2-integrin subunit is inhibited in c-erbB2-transfected human mammary epithelial cells.

Europe PMC ID: 8099725

Bartek et al. 1991. Proc Natl Acad Sci U S A. 88(9):3520-4. PMID: 1708884.

Efficient immortalization of luminal epithelial cells from human mammary gland by introduction of simian virus 40 large tumor antigen with a recombinant retrovirus.

Europe PMC ID: 1708884


Add a reference

References: 6 entries

D'souza et al. 1994. Proc Natl Acad Sci U S A. 91(15):7202-6. PMID: 7913748.

Overexpression of ERBB2 in human mammary epithelial cells signals inhibition of transcription of the E-cadherin gene.

Europe PMC ID: 7913748

D'Souza et al. 1993. Oncogene. 8(7):1797-806. PMID: 8099725.

Collagen-induced morphogenesis and expression of the alpha 2-integrin subunit is inhibited in c-erbB2-transfected human mammary epithelial cells.

Europe PMC ID: 8099725

Bartek et al. 1991. Proc Natl Acad Sci U S A. 88(9):3520-4. PMID: 1708884.

Efficient immortalization of luminal epithelial cells from human mammary gland by introduction of simian virus 40 large tumor antigen with a recombinant retrovirus.

Europe PMC ID: 1708884


Add a reference

References: 6 entries

D'souza et al. 1994. Proc Natl Acad Sci U S A. 91(15):7202-6. PMID: 7913748.

Overexpression of ERBB2 in human mammary epithelial cells signals inhibition of transcription of the E-cadherin gene.

D'Souza et al. 1993. Oncogene. 8(7):1797-806. PMID: 8099725.

Collagen-induced morphogenesis and expression of the alpha 2-integrin subunit is inhibited in c-erbB2-transfected human mammary epithelial cells.

Bartek et al. 1991. Proc Natl Acad Sci U S A. 88(9):3520-4. PMID: 1708884.

Efficient immortalization of luminal epithelial cells from human mammary gland by introduction of simian virus 40 large tumor antigen with a recombinant retrovirus.


Add a reference


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