Anti-APC4 [CIV1.1]
Invented by Dr Jonathon Pines from Gurdon Institute
Invented at University of Cambridge
- Datasheet
- References (4)
- Inventor Info
Info
| Catalogue Number | 151500 |
| Applications | IP WB |
| Antigen/Gene or Protein Targets | Anaphase-promoting complex (APC) 4 |
| Reactivity | Human |
| Relevance | APC4 is a component of the anaphase-promoting complex (APC). The APC complex functions as an ubiquitin ligase which promotes the transition from metaphase to anaphase of the cell cycle. APC4 contains WD repeats and has a molecular weight of approximately 92 kD. APC4 is located in the nucleus during interphase and at the centrosome during metaphase/anaphase. |
| Host | Mouse |
| Immunogen | C-terminus of APC4 |
| Positive Control | Purified APC/C by silver and mass spectrometry |
| Subclass | IgG |
| Molecular Weight (kDa) | 92 |
| Notes |
CIV1.1 may be used for the affinity purification of the Anaphase Promoting Complex (APC/C) by peptide elution. Suggested Dilution for western blot: 1:500. It is suggested to blot for at least 2 hours (wet transfer) to ensure that APC4 is transferred efficiently. Mass spectrometry was used to confirm that the whole APC/C is co-precipitated. To establish the APC4 specific bands, it is suggested to do an APC4 immunoprecipitation assay showing input, IP and FT and blot for at least 2 hours. |
| Research Area | Cell Cycle, Protein Degradation |
References: 4 entries
Di Fiore et al. 2015. Dev Cell. 32(3):358-72. PMID: 25669885.
The ABBA motif binds APC/C activators and is shared by APC/C substrates and regulators.
Europe PMC ID: 25669885
Sedgwick et al. 2013. EMBO J. 32(2):303-14. PMID: 23288039.
IP
Mechanisms controlling the temporal degradation of Nek2A and Kif18A by the APC/C-Cdc20 complex.
Europe PMC ID: 23288039
Add a reference
References: 4 entries
Di Fiore et al. 2015. Dev Cell. 32(3):358-72. PMID: 25669885.
The ABBA motif binds APC/C activators and is shared by APC/C substrates and regulators.
Sedgwick et al. 2013. EMBO J. 32(2):303-14. PMID: 23288039.
IP
Mechanisms controlling the temporal degradation of Nek2A and Kif18A by the APC/C-Cdc20 complex.
Add a reference
