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|Antigen/Gene or Protein Targets||Anaphase-promoting complex (APC) 4|
|Relevance||APC4 is a component of the anaphase-promoting complex (APC). The APC complex functions as an ubiquitin ligase which promotes the transition from metaphase to anaphase of the cell cycle. APC4 contains WD repeats and has a molecular weight of approximately 92 kD. APC4 is located in the nucleus during interphase and at the centrosome during metaphase/anaphase.|
|Immunogen||C-terminus of APC4|
|Positive Control||Purified APC/C by silver and mass spectrometry|
|Molecular Weight (kDa)||92|
CIV1.1 may be used for the affinity purification of the Anaphase Promoting Complex (APC/C) by peptide elution.
Suggested Dilution for western blot: 1:500. It is suggested to blot for at least 2 hours (wet transfer) to ensure that APC4 is transferred efficiently.
Mass spectrometry was used to confirm that the whole APC/C is co-precipitated.
To establish the APC4 specific bands, it is suggested to do an APC4 immunoprecipitation assay showing input, IP and FT and blot for at least 2 hours.
|Research Area||Cell Cycle, Protein Degradation|
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