Cancer Research Technology
Log in Register
Menu

Anti-APC4 [CIV1.1]

Invented by Dr Jonathon Pines at Gurdon Institute

Info

Catalogue Number 151500
Applications IP WB
Antigen/Gene or Protein Targets Anaphase-promoting complex (APC) 4
Reactivity Human
Relevance APC4 is a component of the anaphase-promoting complex (APC). The APC complex functions as an ubiquitin ligase which promotes the transition from metaphase to anaphase of the cell cycle. APC4 contains WD repeats and has a molecular weight of approximately 92 kD. APC4 is located in the nucleus during interphase and at the centrosome during metaphase/anaphase.
Host Mouse
Immunogen C-terminus of APC4
Subclass IgG
Positive Control Purified APC/C by silver and mass spectrometry
Molecular Weight (kDa) 92
Notes CIV1.1 may be used for the affinity purification of the Anaphase Promoting Complex (APC/C) by peptide elution.

Suggested Dilution for western blot: 1:500. It is suggested to blot for at least 2 hours (wet transfer) to ensure that APC4 is transferred efficiently.

Mass spectrometry was used to confirm that the whole APC/C is co-precipitated.

To establish the APC4 specific bands, it is suggested to do an APC4 immunoprecipitation assay showing input, IP and FT and blot for at least 2 hours.
Research Area Cell Cycle, Protein Degradation

References: 4 entries

Di Fiore et al. 2015. Dev Cell. 32(3):358-72. PMID: 25669885.

The ABBA motif binds APC/C activators and is shared by APC/C substrates and regulators.

Europe PMC ID: 25669885

Sedgwick et al. 2013. EMBO J. 32(2):303-14. PMID: 23288039.

IP

Mechanisms controlling the temporal degradation of Nek2A and Kif18A by the APC/C-Cdc20 complex.

Europe PMC ID: 23288039


Add a reference

References: 4 entries

Di Fiore et al. 2015. Dev Cell. 32(3):358-72. PMID: 25669885.

The ABBA motif binds APC/C activators and is shared by APC/C substrates and regulators.

Europe PMC ID: 25669885

Sedgwick et al. 2013. EMBO J. 32(2):303-14. PMID: 23288039.

IP

Mechanisms controlling the temporal degradation of Nek2A and Kif18A by the APC/C-Cdc20 complex.

Europe PMC ID: 23288039


Add a reference

References: 4 entries

Di Fiore et al. 2015. Dev Cell. 32(3):358-72. PMID: 25669885.

The ABBA motif binds APC/C activators and is shared by APC/C substrates and regulators.

Sedgwick et al. 2013. EMBO J. 32(2):303-14. PMID: 23288039.

IP

Mechanisms controlling the temporal degradation of Nek2A and Kif18A by the APC/C-Cdc20 complex.


Add a reference


This reagent does not have any reviews at the moment.