HEK293A EGFP-LC3 Cell Line
Invented by Dr Sharon Tooze from The Francis Crick Institute
Invented at Cancer Research UK London Research Institute: Lincoln's Inn Fields
- Datasheet
- References (4)
- Inventor Info
Info
| Catalogue Number | 151480 |
| Antigen/Gene or Protein Targets | GFP-LC3B (Rat) |
| Parental Line | HEK293 |
| Host | Human |
| Tissue | Embryo |
| Disease Keywords | Cancer, neurodegeneration, infectious diseases |
| Model | Transgenic |
| Relevance | HEK 293A EGFP-LC3 is a stable epithelial cell line expressing EGFP-tagged LC3 (rat) in a HEK293A background. This line functions as a reporter cell line to monitor the induction of autophagy after aminoacid starvation or rapamycin treatment as well as autophagosome formation. This cell line uses GFP-LC3 as a standard assay read out and can be evaluated by both immunofluorescence and biochemical methods. |
| Production Details | EGFP-LC3 (rat) plasmid was transfected into HEK293A cells. The cells were selected with 800ug/ml geneticin. Single cell clones were selected and grown up. Several clones were tested and clone 2GL9 was taken as the best based on a number of parameters (inc. GFP-LC3 expression levels and responsiveness to amino acid deprivation). |
| Research Area | Apoptosis and Programmed Cell Death, Cancer, Cell Signaling & Signal Transduction, Cellular Transport, Drug Discovery & Development, Metabolism |
| Growth/Phenotype Keywords | Autophagy |
| Recommended Growing Conditions | DMEM + 10% FCS + 2mM Glutamine. (400ug/ml geneticin can be added for maintainance but this is not normally necessary as the GFP-LC3 expression level is maintained over at least 20 passages). |
| Notes |
Stable cell line expressing EGFP-tagged LC3 (rat) in background of HEK293A cell line. HEK293 cells were obtained under licence from AdVec |
| Cellosaurus ID | CVCL_W347 |
References: 4 entries
Chan et al. 2007. J Biol Chem. 282(35):25464-74. PMID: 17595159.
siRNA screening of the kinome identifies ULK1 as a multidomain modulator of autophagy.
Europe PMC ID: 17595159
Köchl et al. 2006. Traffic. 7(2):129-45. PMID: 16420522.
Microtubules facilitate autophagosome formation and fusion of autophagosomes with endosomes.
Europe PMC ID: 16420522
Add a reference
References: 4 entries
Chan et al. 2007. J Biol Chem. 282(35):25464-74. PMID: 17595159.
siRNA screening of the kinome identifies ULK1 as a multidomain modulator of autophagy.
Köchl et al. 2006. Traffic. 7(2):129-45. PMID: 16420522.
Microtubules facilitate autophagosome formation and fusion of autophagosomes with endosomes.
Add a reference
