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HEK293A EGFP-LC3 Cell Line

Invented by Dr Sharon Tooze at The Francis Crick Institute

Info

Catalogue Number 151480
Antigen/Gene or Protein Targets GFP-LC3B (Rat)
Parental Line HEK293
Host Human
Tissue Embryo
Disease Keywords Cancer, neurodegeneration, infectious diseases
Model Transgenic
Relevance HEK 293A EGFP-LC3 is a stable epithelial cell line expressing EGFP-tagged LC3 (rat) in a HEK293A background. This line functions as a reporter cell line to monitor the induction of autophagy after aminoacid starvation or rapamycin treatment as well as autophagosome formation. This cell line uses GFP-LC3 as a standard assay read out and can be evaluated by both immunofluorescence and biochemical methods.
Production Details EGFP-LC3 (rat) plasmid was transfected into HEK293A cells. The cells were selected with 800ug/ml geneticin. Single cell clones were selected and grown up. Several clones were tested and clone 2GL9 was taken as the best based on a number of parameters (inc. GFP-LC3 expression levels and responsiveness to amino acid deprivation).
Research Area Apoptosis and Programmed Cell Death, Cancer, Cell Signaling & Signal Transduction, Cellular Transport, Drug Discovery & Development, Metabolism
Growth/Phenotype Keywords Autophagy
Recommended Growing Conditions DMEM + 10% FCS + 2mM Glutamine. (400ug/ml geneticin can be added for maintainance but this is not normally necessary as the GFP-LC3 expression level is maintained over at least 20 passages).
Notes Stable cell line expressing EGFP-tagged LC3 (rat) in background of HEK293A cell line.

HEK293 cells were obtained under licence from AdVec
Cellosaurus ID CVCL_W347

References: 2 entries

Chan et al. 2007. J Biol Chem. 282(35):25464-74. PMID: 17595159.

Köchl et al. 2006. Traffic. 7(2):129-45. PMID: 16420522.


Add a reference

References: 2 entries

Chan et al. 2007. J Biol Chem. 282(35):25464-74. PMID: 17595159.

Köchl et al. 2006. Traffic. 7(2):129-45. PMID: 16420522.


Add a reference

References: 2 entries

Chan et al. 2007. J Biol Chem. 282(35):25464-74. PMID: 17595159.

Köchl et al. 2006. Traffic. 7(2):129-45. PMID: 16420522.


Add a reference


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