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Raf KI D486A Mouse

Info

Catalogue Number 151475
Antigen/Gene or Protein Targets Raf1 D486A (oncogenic mutant)
Disease Keywords Cancer;
Relevance Knockin of oncogenic Raf-1D486A; in vivo study of oncogenic Raf-1 mutant, and Ras signalling
Production Details A Raf-1 targeting vector, encoding Raf-1 with a substitution at residue 486 (D to A) and a loxP flanked resistance cassette, was transfected into 129Ola ES cells. Raf-1 was targeted by homologous recombination with the targeting vector. Correctly targeted ES cells were transfected with a Cre expressing vector to excise the resistance cassette, before injection into C57BL/6 blastocysts. Chimeric offspring were bred with C57BL/6 mice to yield mice heterozygous for the mutant allele.
Conditional No
Mouse Genetic Background/Cross History C57BL/6
Research Area Cancer, Cell Signaling & Signal Transduction, Epigenetics & Nuclear Signalling, Genetic Studies Tools, Neurobiology

References: 2 entries

Kamata et al. 2010. Cancer Res. 70(21):8475-86. PMID: 20978199.

Noble et al. 2008. Mol Cell. 31(6):862-72. PMID: 18922468.


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