Raf KI D486A Mouse
Invented at University Of Leicester
- Datasheet
- References (4)
- Inventor Info
Info
| Catalogue Number | 151475 |
| Antigen/Gene or Protein Targets | Raf1 D486A (oncogenic mutant) |
| Disease Keywords | Cancer; |
| Relevance | Knockin of oncogenic Raf-1D486A; in vivo study of oncogenic Raf-1 mutant, and Ras signalling |
| Production Details | A Raf-1 targeting vector, encoding Raf-1 with a substitution at residue 486 (D to A) and a loxP flanked resistance cassette, was transfected into 129Ola ES cells. Raf-1 was targeted by homologous recombination with the targeting vector. Correctly targeted ES cells were transfected with a Cre expressing vector to excise the resistance cassette, before injection into C57BL/6 blastocysts. Chimeric offspring were bred with C57BL/6 mice to yield mice heterozygous for the mutant allele. |
| Conditional | No |
| Mouse Genetic Background/Cross History | C57BL/6 |
| Research Area | Cancer, Cell Signaling & Signal Transduction, Epigenetics & Nuclear Signalling, Genetic Studies Tools, Neurobiology |
References: 4 entries
Kamata et al. 2010. Cancer Res. 70(21):8475-86. PMID: 20978199.
BRAF inactivation drives aneuploidy by deregulating CRAF.
Europe PMC ID: 20978199
Noble et al. 2008. Mol Cell. 31(6):862-72. PMID: 18922468.
CRAF autophosphorylation of serine 621 is required to prevent its proteasome-mediated degradation.
Europe PMC ID: 18922468
Add a reference
References: 4 entries
Kamata et al. 2010. Cancer Res. 70(21):8475-86. PMID: 20978199.
BRAF inactivation drives aneuploidy by deregulating CRAF.
Noble et al. 2008. Mol Cell. 31(6):862-72. PMID: 18922468.
CRAF autophosphorylation of serine 621 is required to prevent its proteasome-mediated degradation.
Add a reference
