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DR3 KO Mouse

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Catalogue Number 151474
Antigen/Gene or Protein Targets DR3 (Death Domain Receptor 3)
Relevance In vivo study of DR3-induced apoptosis & function; in vivo sstudies of negative T cell selection and development. The DR3-/- mouse exhibits complete knockout of DR3, a death domain-containing tumour necrosis factor receptor. which mediates one of the key regulators of the cell division cycle. Negative selection and anti-CD3-induced apoptosis are significantly impaired in DR3-null mice. In contrast, both superantigen-induced negative selection and positive selection are normal. The pre-T-cell receptor-mediated checkpoint, which is dependent on TNFR signaling, is also unaffected in DR3-deficient mice. These data reveal a nonredundant in vivo role for this TNF receptor family member in the removal of self-reactive T cells in the thymus.
Production Details A DR3 targeting vector, replacing the entire DR3 coding region with a loxP flanked resistance cassette, was transfected into GK129 ES cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. Chimeric offspring were bred with C57BL/6 mice to yield mice heterozygous for the mutant allele.
Conditional No
Growth/Phenotype Keywords Immune abnormalities (T cell development);
Mouse Genetic Background/Cross History C57BL/6
Zygosity Homozygous
Research Area Apoptosis and Programmed Cell Death, Cancer, Cell Cycle, Cell Signaling & Signal Transduction, Genetic Studies Tools, Immunology

References: 2 entries

Twohig et al. 2010. J Neurosci. 30(10):3782-92. PMID: 20220013.

Wang et al. 2001. Mol Cell Biol. 21(10):3451-61. PMID: 11313471.


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Genotyping method PCR
Method protocol Primer information: DR3.2R - GAA AGG ATG AAA CTT GCC TGT TGG (same as R1); DR3.AV1 (neo) – CAT CGC CTT CTA TCG CCT TC; DR3.4F - AGA AGG AGA AAG TCA GTA GGA CCG (same as F1). Band sizes should be 280 bp for wildtype band, 320 bp for knockout band (NOTES: AV1 PRIMER NEEDS TO BE ULTRAPURE) Searches on the 2R and 4F primers flag espin but we were in between the espin and DR3 genes.
Additional notes

Use 1.6% gel to view the bands.

This genotyping protocol was submitted by Eddie Wang. 

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